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  1. Gaur, Rajiv L ; Ren, Kangning ; et al.
    In: PLOS Pathogens, vol 10, iss 9, 2014
    academicJournal
  2. Gaur, Rajiv L ; Ren, Kangning ; et al.
    In: PLOS Pathogens, vol 11, iss 12, 2015
    academicJournal
  3. Martinot, Amanda J. ; Farrow, Mary ; et al.
    2023
    unknown
  4. Hohl, Michael ; Remm, Sille ; et al.
    In: http://infoscience.epfl.ch/record/266896, 2019
    Online academicJournal
  5. Hohl, Michael ; Remm, Sille ; et al.
    2019
    academicJournal
  6. Figure 2. CwlM is phosphorylated and cytoplasmic. ; (A) 2-D gel of CwlM-FLAG from Msmeg (CB100) with the isoelectric point (pI) indicated on the bottom. (B) Weblogo diagrams of the C-terminus of CwlM homologues. Acetylation (Ac) and phosphorylation (PO4) sites are indicated. The colors of the three phosphates match the colors of the corresponding phospho-ablative strains in C, E and F. (C) Doubling times of L5::cwlM-FLAG WT (CB300) and phospho-ablative mutants (T374A = CB300; T376A = CB345; T378A = CB348). All multiple comparisons throughout were performed by One-way ANOVA with a Dunnett multiplicity correction. The adjusted p values for each mutant compared to WT are: T374A =< 0.0001; T376A = 0.88; T378A = 0.15. (D) α-strep western blot of L5::cwlM-strep WT (CB663) and phospho-ablative mutants (CB666, 669, 672). A background band on the same blot probed with α-FLAG is used as a control. Westerns of the L5::cwlM-FLAG strains could not be used because of high background. (E) Cell length of 200–300 cells from L5::cwlM-FLAG WT and phospho-ablative mutants. The adjusted p values for each mutant compared to WT are: T374A =< 0.0001; T376A = 0.99; T378A = 0.01. (F) Length of the longer unstained pole of cells in (E). Cells were stained with ARD and grown for three hours before imaging. The adjusted p values for each mutant compared to WT are: T374A =< 0.0001; T376A = 0.59; T378A = 0.36. (G) Substituted cysteine accessibility. α-strep western of cells with strep-tagged Pgm (CB886, MSMEG_4579, cytoplasmic control), lprG (CB706, Rv1411, periplasmic control) and CwlM1cys (CB457) that were cysteine-blocked with MTSEA (membrane permeable) or MTSET (membrane impermeable) then alkylated at unblocked cysteines. Control samples: (−) = not alkylated or blocked, (+) = alkylated but not blocked. % alkylation (%alk.) = high band/ total for each sample. The periplasmic localization score = (%alk.MTSEA/% alk.MTSET) and is indicated below the gel for each protein ± the 95% confidence interval from two biological replicates. Scores < 0.5 are cytoplasmic, near 1 are periplasmic.
    2016
    unknown
  7. Seeliger, Jessica ; Shum, Rebecca ; et al.
    2020
    unknown
  8. 2020
    unknown
  9. 2020
    unknown
  10. 2020
    unknown
  11. Martinot, Amanda J. ; Farrow, Mary ; et al.
    2016
    Online unknown
  12. Sznajd, Jozef
    In: Journal of Statistical Mechanics: Theory and Experiment ; volume 2024, issue 5, page 053202 ; ISSN 1742-5468, 2024
    academicJournal
  13. Sznajd, Jozef
    2024
    report
  14. Sznajd, J.
    2013
    academicJournal
  15. Ishwarlall, Tamara Z. ; Adeleke, Victoria T. ; et al.
    In: Frontiers in Immunology ; volume 13 ; ISSN 1664-3224, 2022
    academicJournal
  16. Carroll, Maria V. ; Sim, Robert B. ; et al.
    2010
    academicJournal
  17. Ishwarlall, Tamara Z. ; Adeleke, Victoria T. ; et al.
    2022
    unknown
  18. Haugh, Michael ; Kádár, Daniel Z. ; et al.
    2013
    academicJournal
  19. Luthra, Amit ; Zhu, Guangyu ; et al.
    In: Alejandro P. Heuck, 2011
    Online academicJournal
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