Zum Hauptinhalt springen

Suchergebnisse

UB Katalog
Ermittle Trefferzahl…

Artikel & mehr
745 Treffer

Suchmaske

Suchtipp für den Bereich Artikel & mehr: Wörter werden automatisch mit UND verknüpft. Eine ODER-Verknüpfung erreicht man mit dem Zeichen "|", eine NICHT-Verknüpfung mit einem "-" (Minus) vor einem Wort. Anführungszeichen ermöglichen eine Phrasensuche.
Beispiele: (burg | schloss) -mittelalter, "berufliche bildung"

Das folgende Suchfeld wird hier nicht unterstützt: "Signatur / Strichcode".

Suchergebnisse einschränken oder erweitern

Erscheinungszeitraum

Mehr Treffer

Weniger Treffer

Gefunden in

Art der Quelle

Schlagwort

Verlag

Publikation

Geographischer Bezug

745 Treffer

Sortierung: 
  1. Konishi, E. ; Minorikawa, Y. ; et al.
    2007
    academicJournal
  2. In: Encyclopedia of Biophysics ; page 2166-2166; (2013)
    Buch
  3. In: Postconference Digest Quantum Electronics and Laser Science, 2003. QELS, 2003
    Online Konferenz
  4. In: 2007 Quantum Electronics and Laser Science Conference, 2007
    Online Konferenz
  5. In: 2007 Conference on Lasers and Electro-Optics (CLEO, 2007
    Online Konferenz
  6. In: 2007 Conference on Lasers and Electro-Optics (CLEO, 2007
    Online Konferenz
  7. In: 2007 Conference on Lasers and Electro-Optics (CLEO, 2007
    Online Konferenz
  8. In: 2007 Conference on Lasers and Electro-Optics (CLEO, 2007
    Online Konferenz
  9. In: 2007 Conference on Lasers and Electro-Optics (CLEO, 2007
    Online Konferenz
  10. In: 2005 Quantum Electronics and Laser Science Conference, 2005
    Online Konferenz
  11. Briët, M. ; Näring, G.W.B. ; et al.
    In: Gedrag & Gezondheid, 33, 5, pp. 318-330, 2005
    Online academicJournal
  12. In: ISBN 1557527490, 2003
    Konferenz
  13. Figure 1—figure supplement 3. The TCP domain of CPAP is predominantly monomeric in solution. ; (A) Panel showing size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) chromatograms. CPAP937–1124 was injected at concentrations of approximately 70 μM (light grey), 460 μM (dark grey) and 2.4 mM (black). The corresponding chromatogram traces (thin lines) show the refractive index signal. The concentrations of the CPAP937–1124 monomer measured at the peaks are indicated, and heavy solid lines across the peaks show the calculated molar masses. When averaged across the central 50% of the peaks, these molar masses were 22 kDa, 23 kDa, and 29 kDa, respectively. The theoretical molecular weight of a CPAP937–1124 monomer is 22 kDa. The Rh of CPAP937–1124 determined at the intermediate concentration was 2.9 ± 0.15 nm, which is significantly larger than expected for a globular protein of this mass and thus is consistent with the extended crystallographic structure. SEC-MALS measurements were performed using a Wyatt Heleos II 18 angle light scattering instrument coupled to a Wyatt Optilab rEX online refractive index detector. Detector 12 in the Heleos instrument was replaced with Wyatt's QELS detector for dynamic light scattering measurement. Samples (100 μl) were resolved on a Superdex S-200 10/300 analytical gel filtration column (GE Healthcare, Little Chalfont, UK) running at 0.5 ml/min in 25 mM bis Tris pH 7.2, 100 mM NaCl buffer before passing through the light scattering and refractive index detectors in a standard SEC-MALS format. Protein concentration was determined from the excess differential refractive index based on 0.186 RI increment for 1 g/ml protein solution. The concentration and the observed scattered intensity at each point in the chromatograms were used to calculate the absolute molecular mass from the intercept of the Debye plot using Zimm's model as implemented in Wyatt's ASTRA software. Autocorrelation analysis of data from the dynamic light scattering detector was also performed using Wyatt's ASTRA software, and the translational diffusion coefficients determined were used to calculate the hydrodynamic radius using the Stokes-Einstein equation and the measured solvent viscosity of 9.3 e-3 Poise. (B) Small-angle X-ray scattering (SAXS) experiment with approximately 20 μM D. rerio CPAP937–1124 in 25 mM bis Tris pH 7.2, 100 mM NaCl, 2 mM DTT. Shown in blue is the experimentally measured SAXS curve of CPAP937–1124 with the experimental error indicated by black bars. The orange line shows the fitted theoretical SAXS curve of CPAP937–1124 derived from its crystal structure. Fitting was done using CRYSOL (Svergun et al., 1995) and resulted in a χ-value of 1.416. A.U., arbitrary units. At higher CPAP937–1124 concentrations the fit became less good due to the tendency of CPAP937–1124 to self-associate at these concentrations as revealed by a gradual increase of the derived Rg values. SAXS data were collected at the European Synchrotron Radiation Facility (ESRF), Grenoble, France, at beamline ID14–3. Measurements were done at 10°C at a wavelength of 0.931 Å with the standard beamline settings using a PILATUS 1M detector (Dectris, Baden, Switzerland). To minimise radiation damage, a flow cell was used for the measurements. Collected data was buffer subtracted using PRIMUS (Konarev et al., 2003) and the beamstop shadow removed by cutting the data at a q-value of 0.055 nm−1. Above a q-value of 3.8 nm−1 the data became too noisy to be interpretable and the data were therefore cut at this value.
    2013
    unknown
  14. Ivanova, Mariya A. ; Klopov, Nicolay V. ; et al.
    In: SPIE Proceedings ; Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology ; ISSN 0277-786X, 1997
    Konferenz
  15. Lebedev, Andrei D. ; Ivanova, Mariya A. ; et al.
    In: SPIE Proceedings ; Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology ; ISSN 0277-786X, 1997
    Konferenz
  16. Earnshaw, J. C. ; Nugent, C. P. ; et al.
    In: SPIE Proceedings ; Static and Dynamic Light Scattering in Medicine and Biology ; ISSN 0277-786X, 1993
    Konferenz
  17. Hensinger, Winfried K
    2001
    academicJournal
  18. Hensinger, Winfried
    2001
    academicJournal
  19. DAPRANO, A ; JANNELLI, MP ; et al.
    1996
    Online academicJournal
  20. 2013
    unknown
xs 0 - 576
sm 576 - 768
md 768 - 992
lg 992 - 1200
xl 1200 - 1366
xxl 1366 -