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  1. Hui, C. Y. ; Seo, K. A. ; et al.
    2012
    academicJournal
  2. Figure 3. C-di-GMP binds to C-terminal Arg-rich peptides. ; (a) c-di-GMP binding to CleD requires the ARR. Microscale thermophoresis (MST) experiments with purified full-length His-CleD and truncated variants using 60 nM of the ligand 2'-Fluo-AHC-c-di-GMP (flr-cdG). Kds were determined as follows: 1.2 µM for His-CleD (aa 19–269), 1.3 µM for His-CleD (aa 19–262), 6.2 µM for His-CleD-rec-ARR (aa 19–174) and 41.7 µM for His-CleD-rec (aa 19–141). (b) The ARR peptide is sufficient to bind c-di-GMP. The ARR of CleD was fused to the C-terminus of E. coli CheY. Binding of flr-cdG to purified His-CheY (green) and its variants containing a wild-type ARR (red) or the R156A mutant ARR (blue) was determined by MST. Apparent Kds were calculated as 14.8 µM (red) and 471 µM (blue). (c) c-di-GMP binds to the ARR peptides of all five Cle proteins. MST experiments were done as outlined above. The ARR peptide regions fused to His-SUMO are indicated in Figure 1c. Binding was determined to His-SUMO (grey), and to His-SUMO with fused ARR regions of CleB (pink), CleC (light blue), CleD (red) and CleE (dark blue), or with the ARR-CTR region of CleA (dark green). The respective Kd values are indicated. (d) Sequence logo of the alignment generated by Jackhmmer when queried with the ARR region of CleD. Analysis of a representative pool of sequences (see Materials and Methods) identified the tandem motif [YF]XGP[DE][RK]R. The overall height of the stack indicates the sequence conservation at that position, while the height of symbols within the stack indicates the relative frequency of each amino acid at that position. Bits: Information content in bits.
    2017
    unknown
  3. Weissman, Simcha ; Ghaffar, Madeeha ; et al.
    In: Touro College of Osteopathic Medicine (New York) Publications and Research, 2018
    Online academicJournal
  4. Mishriki, Yehia Y, MD ; Doehne, Karin
    In: Department of Medicine, 2010
    academicJournal
  5. Mirza, Muhammad Usman ; Taunay, John Van ; et al.
    In: Medical College Documents, 2019
    Online academicJournal
  6. Hands, A. D. P. ; Warwick, R. S. ; et al.
    2004
    academicJournal
  7. Hands, A. ; Warwick, R. ; et al.
    2002
    academicJournal
  8. A. Artiles-Medina (10537531) ; I. Laso-García (10537534) ; et al.
    2021
    academicJournal
  9. Petca, Răzvan C. ; Popescu, Răzvan I. ; et al.
    In: Journal of Mind and Medical Sciences, 2019
    academicJournal
  10. Shahrestani, Jasmin
    In: Health Professions Division REACH Day, 2020
    Bild
  11. BARTOLI, F ; NIGLIO, F ; et al.
    2007
    Online academicJournal
  12. Fisher, Ann F. ; Dembry, Louise M. ; et al.
    In: Schlossberg's Clinical Infectious Disease ; page 433-438; (2021)
    Buch
  13. Khaira, Herkanwal S. ; Shah, Rajal B. ; et al.
    2005
    Online academicJournal
  14. Zaid, Uwais B ; Porten, Sima P ; et al.
    2013
    academicJournal
  15. Hands, A. D. P. ; Warwick, R. S. ; et al.
    2004
    Zeitungsartikel
  16. Desai, Devang J.
    2017
    academicJournal
  17. Onder, ENA ; Ozkol, M ; et al.
    Online unknown
  18. Hands, Alex.
    2004
    Hochschulschrift
  19. Phillips, George
    In: George Phillips Photograph Collection, 1959
    unknown
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