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112 Treffer

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  1. Detailansicht für Treffer 1 öffnen
    Development of the XFP beamline for x-ray footprinting at NSLS-II
    Bohon, Jen ; Sullivan, Michael ; et al.
    In: AIP Conference Proceedings ; ISSN 0094-243X, 2016
    Konferenz
    Wie komme ich dran?
  2. Detailansicht für Treffer 2 öffnen
    Allosteric Regulation of Bacterial and Fungal Xylulose 5-phosphate/ Fructose 6-phosphate Phosphoketolases (Xfps)
    Glenn, Katie
    In: All Dissertations, 2014
    academicJournal
    Wie komme ich dran?
  3. Detailansicht für Treffer 3 öffnen
    The XFP (17-BM) beamline for X-ray footprinting at NSLS-II
    Asuru, Awuri ; Farquhar, Erik R. ; et al.
    2023
    unknown
    Wie komme ich dran?
  4. Detailansicht für Treffer 4 öffnen
    The XFP (17-BM) beamline for X-ray footprinting at NSLS-II
    Asuru, Awuri ; Farquhar, Erik R. ; et al.
    2023
    unknown
    Wie komme ich dran?
  5. Detailansicht für Treffer 5 öffnen
    The XFP (17-BM) beamline for X-ray footprinting at NSLS-II
    Asuru, Awuri ; Farquhar, Erik R. ; et al.
    2023
    unknown
    Wie komme ich dran?
  6. Detailansicht für Treffer 6 öffnen
    The XFP (17-BM) beamline for X-ray footprinting at NSLS-II
    Asuru, Awuri ; Farquhar, Erik R. ; et al.
    2023
    unknown
    Wie komme ich dran?
  7. Detailansicht für Treffer 7 öffnen
    The XFP (17-BM) beamline for X-ray footprinting at NSLS-II
    Asuru, Awuri ; Farquhar, Erik R. ; et al.
    2023
    unknown
    Wie komme ich dran?
  8. Detailansicht für Treffer 8 öffnen
    Figure 3—figure supplement 1. Tetbow optimization. ; We tried to enhance the expression levels of XFPs by adding WPRE to the 3’′-UTR of the tTA gene, but the expression levels of XFPs were decreased with WPRE. The imaging conditions were the same as for Figure 2.
    2018
    unknown
    Wie komme ich dran?
  9. Detailansicht für Treffer 9 öffnen
    Figure 2—figure supplement 1. Tetbow constructs. ; Plasmid maps for CAG-tTA, TRE-mTurquoise2/EYFP/tdTomato. To ensure adequate plasmid yield in Escherichia coli, the TRE expression cassette was transferred to a high-copy plasmid, pBluescriptII (SK+). To enhance the stability of mRNA, a WPRE sequence is added to the 3’’ UTR. We used cytosolic XFPs, because cytosolic XFPs are much brighter than membrane localized XFPs (data not shown).
    2018
    unknown
    Wie komme ich dran?
  10. Detailansicht für Treffer 10 öffnen
    Figure 2—figure supplement 2. Comparison with Brainbow. ; In utero electroporation was used to label M/T cells at E12 and mice were analyzed at P8. Whole brains were cleared with SeeDB2G and imaged with confocal microscopy. Low and high magnification images in axons of M/T cells are shown. High magnification images show the axon collaterals in the piriform cortex. (A) Tetbow constructs were introduced. Maximal intensity projection images of confocal images are shown (left: 1330.11 μm thick, right: 23.97 μm thick). (B) CAG-iCre (0.1 μg/μL), EF1a-BbTagBY (0.5 μg/μL), and EF1a-BbChT (0.5 μg/μL) were introduced. In utero electroporation was performed at E15 and mice were analyzed at P21. Experiments were performed in parallel, and image acquisition conditions were the same in (A) and (B). Note, however, that the Brainbow constructs use an EF1a promoter and membrane-bound XFPs, whereas Tetbow uses a TRE promoter and cytosolic XFPs. We also tested the CAG-Brainbow3.0 in the cerebral cortex, but labeling signals were much lower than Tetbow (data not shown). Scale bars are 1 mm (left) and 20 μm (right). Three samples were imaged in the same condition. A representative sample is shown for (A). The brightest sample is shown in (B), as we could not find clear signals at axons in the two remaining samples after clearing. OB, olfactory bulb; OT, olfactory tubercle, PC, piriform cortex.
    2018
    unknown
    Wie komme ich dran?
  11. Detailansicht für Treffer 11 öffnen
    Figure 1. Tetbow strategy. ; Stochastic representation of the vector-mediated multicolor labeling strategy. Here we assume that the copy numbers of introduced vectors follow a Poisson distribution. In a plasmid or virus-mediated gene transfer method, we do not need to use the Cre-loxP system. Rather, it is important to limit the number of genes introduced into each cell. For example, in theory, an average of 2 copies/cell/color can result in the stochastic expression of three different genes (B). If we introduce a copy number that is too small (e.g. an average of 0.2 copies/cell; A), only one of the three genes will be expressed in most cells. If too many genes are introduced in each cell (e.g. an average of 20 copies/cell; C), color variations will be reduced because many of the neurons will express a similar number of XFP genes. The use of the Cre-loxP system alone cannot solve this problem. Simulations of the expected color variations that are based on the various Poisson distributions (middle) are shown as ternary plots (100 plots/condition, right panel). The numerical data are presented in Figure 1—source data 1. Note that many of the plots are overlapping at the edge of the triangle in (A). Three different XFPs are shown in red (R), green (G), and blue (B). (D) The difference between two colors represented as Euclidean distance in 3D color space. For example, a difference between colors a and b is represented as the distance, d. (E) Box plots of Euclidean distances in all pairs of plots in 3D space in relation to copy number. The horizontal lines within each box represents the median, the box represents the interquartile range, and the whiskers show the minimum and maximum values. (F) A cartoon illustrating the cells inside (X%) and outside the threshold Euclidean distance. The cells outside (100 – X %) are considered to be discernable. (G) The percentage of discernable cells for each threshold d and each copy number. Simulation data used for (E) and (G) are provided in Figure 1—source data 1. (H) The color discrimination abilities of experienced researchers. Tests are explained in Figure 1—figure supplement 1. Mean ± SD are indicated in red. When threshold d is 0.1, the score was 94.5 ± 1.73% (mean ± SD). Score data are in Figure 1—source data 2.
    2018
    unknown
    Wie komme ich dran?
  13. Detailansicht für Treffer 13 öffnen
    Figure 6—figure supplement 1. Plasmid maps for chemical-tag Tetbow. ; Plasmid maps for a chemical-tag Tetbow. Chemical-tag expression cassettes are basically the same as those for XFPs.
    2018
    unknown
    Wie komme ich dran?
  14. Detailansicht für Treffer 14 öffnen
    Figure 4. Tetbow labeling is bright enough for high-resolution imaging of synaptic structures. ; Volume rendering of Layer 2/3 cortical pyramidal neurons labeled with Tetbow (P70). In utero electroporation was used to label L2/3 neurons at E15. Brain slices were cleared with SeeDB2G and imaged with confocal microscopy. Different neurons were brightly labeled with different colors. Representative images are shown from four independent experiments. (A) Low- and high-magnification images in Layer 2/3 (45.16 μm thick). The four panels on the right indicate each of the three single channel fluorescence images. See also Video 1. (B) Dendrites and dendritic spines were brightly labeled with various colors with Tetbow. A volume-rendered image (18.65 μm thick) is shown. See also Video 2. (C) Axons and axonal boutons were clearly visualized with Tetbow. A volume rendered image (23.01 μm thick) is shown. Note that the synaptic-scale structures were clearly visualized with the native fluorescence of XFPs, without antibody staining. Scale bars are 20 μm (A, left) and 5 μm (A, right and B, C).
    2018
    unknown
    Wie komme ich dran?
  15. Detailansicht für Treffer 15 öffnen
    Figure 3. Optimization of Tetbow plasmid concentrations for in utero electroporation. ; (A–C) Plasmid concentrations for the four Tetbow vectors (CAG-tTA and three TRE-XFP vectors) were tested at 0.05, 0.1, 0.25, and 0.5 μg/μL. (B) Boxplot of median EYFP fluorescence intensities (normalized to the median of Tetbow (0.05 µg/µl)). A D’Agostino and Pearson normality test showed that the data were not normally distributed (p
    2018
    unknown
    Wie komme ich dran?
  16. Detailansicht für Treffer 16 öffnen
    Figure 7—figure supplement 1. AAV vector maps. ; Plasmid maps for the pAAV vectors used in this study. TRE-XFP expression cassettes are basically the same as in in utero electroporation. The tTA gene was expressed under the SYN1 promoter to ensure neuronal expression.
    2018
    unknown
    Wie komme ich dran?
  19. Detailansicht für Treffer 19 öffnen
    Investigating optimal regimes of operation for pluggable XFPs in multi-channels 10G systems exploiting nonlinear effects
    Atieh, Ahmad ; Kemp, Josh P. ; et al.
    In: Optical Transmission Systems, Subsystems, and Technologies IX ; SPIE Proceedings ; ISSN 0277-786X, 2011
    Konferenz
    Wie komme ich dran?
  20. Detailansicht für Treffer 20 öffnen
    Investigating optimal regime of operation for 10G optical communication systems using pluggable XFPs
    Atieh, Ahmad ; Copping, Philip ; et al.
    In: SPIE Proceedings ; Photonics North 2010 ; ISSN 0277-786X, 2010
    Konferenz
    Wie komme ich dran?
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