Creation of Functionally Diverse Chimerical α-Glucosidase Enzymes by Swapping Homologous Gene Fragments Retrieved from Soil DNA.
In: Indian Journal of Microbiology, Jg. 55 (2015-03-01), Heft 1, S. 114-117
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Zugriff:
α-Glucosidase (XcG) from Xanthomonas campestris is an interesting enzyme due to its ability to catalyze transglycosylation reactions using maltose, rather than expensive nucleotide-activated sugars. In this study, two chimerical enzymes, XcG-A and XcG-B, were created by substituting the corresponding region of the XcG gene with gene fragments retrieved from metagenomic DNA samples. The enzymatic characterization results revealed that XcG-A exhibited a significant greater transglycosylation capacity for α-arbutin production and a significantly improved ability to produce α-arbutin-α-glycosides than XcG and XcG-B. [ABSTRACT FROM AUTHOR]
Titel: |
Creation of Functionally Diverse Chimerical α-Glucosidase Enzymes by Swapping Homologous Gene Fragments Retrieved from Soil DNA.
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Autor/in / Beteiligte Person: | Wang, Qiuyan ; Yin, Xiaopu ; Ma, Lifang ; Pei, Xiaolin ; Du, Pengfei ; Li, Chenglu ; Xie, Tian ; Yu, Lei ; Yu, Li |
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Zeitschrift: | Indian Journal of Microbiology, Jg. 55 (2015-03-01), Heft 1, S. 114-117 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 0046-8991 (print) |
DOI: | 10.1007/s12088-014-0493-5 |
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