Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose.
In: Journal of Biotechnology, Jg. 195 (2015-02-10), S. 82-88
academicJournal
Zugriff:
Production of poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) with Ralstonia eutropha relies on the addition of propionate during fermentation, and propionate consumption is one of the major factors affecting the cost of PHBV production. In this study, 7 strains were obtained by genetic manipulating the methylcitric acid cycle and the methylmalonyl-CoA pathway in R. eutropha . Disruption of prpC1 and prpC2 genes did not affect cell growth and PHBV accumulation. All 7 strains were able to accumulation high amounts of PHBVs with 3HV fractions of 0.41–29.1 mol% during cultivation in flasks. Fermentation in 7.5-L fermenter showed that genetically engineered Rem-8 was able to yield biomass of 132.8 CDW g/L, of which 68.6% were PHBV with 3HV fraction of 26.0 mol% in the biopolymer, indicating promising potentials of commercialization in the future. [ABSTRACT FROM AUTHOR]
Titel: |
Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose.
|
---|---|
Autor/in / Beteiligte Person: | Zhang, Ying-Zi ; Liu, Gui-Ming ; Weng, Wei-Qi ; Ding, Jiu-Yuan ; Liu, Shuang-Jiang |
Link: | |
Zeitschrift: | Journal of Biotechnology, Jg. 195 (2015-02-10), S. 82-88 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 0168-1656 (print) |
DOI: | 10.1016/j.jbiotec.2014.12.014 |
Schlagwort: |
|
Sonstiges: |
|