High-Frequency Secondary Mutations after Suicide-Driven Allelic Exchange Mutagenesis in Extraintestinal Pathogenic Escherichia coli.
In: Journal of Bacteriology, Jg. 185 (2003-09-01), Heft 17, S. 5301-5305
Online
academicJournal
Zugriff:
Frequent unintended secondary mutations occurred in extraintestinal pathogenic Escherichia coli strains CP9, CFT073, and RS218 during suicide plasmid-mediated, putatively specific deletions of hlyA, papG allele III, and iha. Pulsed-field gel electrophoresis and PCR analyses demonstrated genomic alterations and/or unintended loss of defined virulence genes (papG, the F7-2 papA allele, iutA, sat, hlyD, and cnf). Caution is warranted when attributing the observed phenotypic changes to the intended mutation. [ABSTRACT FROM AUTHOR]
| Titel: |
High-Frequency Secondary Mutations after Suicide-Driven Allelic Exchange Mutagenesis in Extraintestinal Pathogenic Escherichia coli.
|
|---|---|
| Autor/in / Beteiligte Person: | Johnson, James R. ; Lockman, Hank A. ; Owens, Krista ; Jelacic, Srdjan ; Tarr, Philip I. |
| Link: | |
| Zeitschrift: | Journal of Bacteriology, Jg. 185 (2003-09-01), Heft 17, S. 5301-5305 |
| Veröffentlichung: | 2003 |
| Medientyp: | academicJournal |
| ISSN: | 0021-9193 (print) |
| DOI: | 10.1128/JB.185.17.5301-5305.2003 |
| Schlagwort: |
|
| Sonstiges: |
|