Failure to launch: the self-regulating Md- MYB10 gene from apple is active in flowers but not leaves of Petunia.
In: Plant Cell Reports, Jg. 34 (2015-10-01), Heft 10, S. 1817-1823
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Key message: The Md - MYB10 gene from apple is capable of self-regulating in heterologous host species and enhancing anthocyanin pigmentation, but the activity of MYB10 is dependent on endogenous protein partners. Abstract: Coloured foliage due to anthocyanin pigments (bronze/red/black) is an attractive trait that is often lacking in many bedding, ornamental and horticultural plants. Apples ( Malus × domestica) containing an allelic variant of the anthocyanin regulator, Md- MYB10, are highly pigmented throughout the plant, due to autoregulation by MYB10 upon its own promoter. We investigated whether Md- MYB10 from apple is capable of functioning within the heterologous host Petunia hybrida to generate plants with novel pigmentation patterns. The Md- MYB10 transgene ( MYB10- R6 :MYB10:MYB10) activated anthocyanin synthesis when transiently expressed in Antirrhinum rosea petals and petunia leaf discs. Stable transgenic petunias containing Md- MYB10 lacked foliar pigmentation but had coloured flowers, complementing the an2 phenotype of 'Mitchell' petunia. The absence of foliar pigmentation was due to the failure of the Md- MYB10 gene to self-activate in vegetative tissues, suggesting that additional protein partners are required for Md-MYB10 to activate target genes in this heterologous system. In petunia flowers, where endogenous components including MYB-bHLH-WDR (MBW) proteins were present, expression of the Md- MYB10 promoter was initiated, allowing auto-regulation to occur and activating anthocyanin production. Md-MYB10 is capable of operating within the petunia MBW gene regulation network that controls the expression of the anthocyanin biosynthesis genes, AN1 (bHLH) and MYBx (R3-MYB repressor) in petals. [ABSTRACT FROM AUTHOR]
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Failure to launch: the self-regulating Md- MYB10 gene from apple is active in flowers but not leaves of Petunia.
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Autor/in / Beteiligte Person: | Boase, Murray ; Brendolise, Cyril ; Wang, Lei ; Ngo, Hahn ; Espley, Richard ; Hellens, Roger ; Schwinn, Kathy ; Davies, Kevin ; Albert, Nick |
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Zeitschrift: | Plant Cell Reports, Jg. 34 (2015-10-01), Heft 10, S. 1817-1823 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 0721-7714 (print) |
DOI: | 10.1007/s00299-015-1827-4 |
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