DAG/PKCδ and IP3/Ca<superscript>2+</superscript>/CaMK IIβ Operate in Parallel to Each Other in PLCγ1-Driven Cell Proliferation and Migration of Human Gastric Adenocarcinoma Cells, through Akt/mTOR/S6 Pathway.
In: International Journal of Molecular Sciences, Jg. 16 (2015-12-01), Heft 12, S. 28510-28522
Online
academicJournal
Zugriff:
Phosphoinositide specific phospholipase Cγ (PLCγ) activates diacylglycerol (DAG)/ protein kinase C (PKC) and inositol 1,4,5-trisphosphate (IP3)/Ca2+/calmodulin-dependent protein kinase II (CaMK II) axes to regulate import events in some cancer cells, including gastric adenocarcinoma cells. However, whether DAG/PKCδ and IP3/Ca2+/CaMK IIβ axes are simultaneously involved in PLC1-driven cell proliferation and migration of human gastric adenocarcinoma cells and the underlying mechanism are not elucidated. Here, we investigated the role of DAG/PKCδ or CaMK IIβ in PLCγ1-driven cell proliferation and migration of human gastric adenocarcinoma cells, using the BGC-823 cell line. The results indicated that the inhibition of PKCδ and CaMK IIβ could block cell proliferation and migration of BGC-823 cells as well as the effect of inhibiting PLCγ1, including the decrease of cell viability, the increase of apoptotic index, the down-regulation of matrix metalloproteinase (MMP) 9 expression level, and the decrease of cell migration rate. Both DAG/PKCδ and CaMK IIβ triggered protein kinase B (Akt)/mammalian target of rapamycin (mTOR)/S6 pathway to regulate protein synthesis. The data indicate that DAG/PKCδ and IP3/Ca2+/CaMK IIβ operate in parallel to each other in PLCγ1-driven cell proliferation and migration of human gastric adenocarcinoma cells through Akt/mTOR/S6 pathway, with important implication for validating PLCγ1 as a molecular biomarker in early gastric cancer diagnosis and disease surveillance. [ABSTRACT FROM AUTHOR]
Titel: |
DAG/PKCδ and IP3/Ca<superscript>2+</superscript>/CaMK IIβ Operate in Parallel to Each Other in PLCγ1-Driven Cell Proliferation and Migration of Human Gastric Adenocarcinoma Cells, through Akt/mTOR/S6 Pathway.
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Autor/in / Beteiligte Person: | Dai, Lianzhi ; Zhuang, Luhua ; Zhang, Bingchang ; Wang, Fen ; Chen, Xiaolei ; Xia, Chun ; Zhang, Bing |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 16 (2015-12-01), Heft 12, S. 28510-28522 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 1661-6596 (print) |
DOI: | 10.3390/ijms161226116 |
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