Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine.
In: Biochemical & Biophysical Research Communications, Jg. 470 (2016-01-29), Heft 1, S. 88-93
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Zugriff:
The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19 kDa protein (nanoKAZ) was determined at 1.71 Å resolution. The structure consists of 11 antiparallel β-strands forming a β-barrel that is capped by 4 short α-helices. The structure of nanoKAZ is similar to those of fatty acid-binding proteins (FABPs), even though the amino acid sequence similarity was very low between them. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the β-barrel structure. [ABSTRACT FROM AUTHOR]
Titel: |
Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine.
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Autor/in / Beteiligte Person: | Tomabechi, Yuri ; Hosoya, Takamitsu ; Ehara, Haruhiko ; Sekine, Shun-ichi ; Shirouzu, Mikako ; Inouye, Satoshi |
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Zeitschrift: | Biochemical & Biophysical Research Communications, Jg. 470 (2016-01-29), Heft 1, S. 88-93 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 0006-291X (print) |
DOI: | 10.1016/j.bbrc.2015.12.123 |
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