Mutation of the RGD sequence does not affect plasma membrane association and growth inhibitory effects of elevated IGFBP-2 in vivo
In: FEBS Letters, Jg. 523 (2002-07-17), Heft 1-3, S. 63-67
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Zugriff:
Using insulin-like growth factor-binding protein-2 (IGFBP-2) transgenic mice (D mice) as a model of elevated IGFBP-2 expression, which is often found in unphysiological conditions, we found association of IGFBP-2 to purified plasma membranes of many organs. To determine whether the RGD (Arg-Gly-Asp) motif of IGFBP-2 mediates cell surface binding in vivo, we mutated the RGD motif of IGFBP-2 into an RGE (Arg-Gly-Glu) sequence and produced transgenic mice (E mice) which express elevated amounts of mutated IGFBP-2. Our data demonstrate that in vivo IGFBP-2 cell surface association is not dependent on the RGD motif and that mutation of this sequence does not alter growth inhibitory effects of IGFBP-2. [Copyright &y& Elsevier]
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Mutation of the RGD sequence does not affect plasma membrane association and growth inhibitory effects of elevated IGFBP-2 in vivo
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Autor/in / Beteiligte Person: | Hoeflich, A. ; Reisinger, R. ; Vargas, G.A. ; Elmlinger, M.W. ; Schuett, B. ; Jehle, P.M. ; Renner-Müller, I. ; Lahm, H. ; Russo, V.C. ; Wolf, E. |
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Zeitschrift: | FEBS Letters, Jg. 523 (2002-07-17), Heft 1-3, S. 63-67 |
Veröffentlichung: | 2002 |
Medientyp: | academicJournal |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/S0014-5793(02)02935-6 |
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