Expression and Regulation of a Silent Operon, hyf, Coding for Hydrogenase 4 Isoenzyme in Escherichia coli.
In: Journal of Bacteriology, Jg. 186 (2004-01-15), Heft 2, S. 580-587
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Zugriff:
On the basis of hyf-lacZ fusion studies, the hyf operon of Escherichia coli, noted for encoding the fourth hydrogenase isoenzyme (HYD4), is not expressed at a significant level in a wild-type strain. However, mutant FhlA proteins (constitutive activators of the hyc-encoded hydrogenase 3 isoenzyme) activated hyf-lacZ. HyfR, an FhlA homolog encoded by the hyfR gene present at the end of the hyf operon, also activated transcription of hyf-lacZ but did so only when hyfR was expressed from a heterologous promoter. The HYD4 isoenzyme did not substitute for HYD3 in H[sub 2] production. Optimum expression of hyf-lacZ required the presence of cyclic AMP receptor protein-cyclic AMP complex and anaerobic conditions when HyfR was the activator. [ABSTRACT FROM AUTHOR]
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Expression and Regulation of a Silent Operon, hyf, Coding for Hydrogenase 4 Isoenzyme in Escherichia coli.
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Autor/in / Beteiligte Person: | Self, William T. ; Hasona, Adnan ; Shanmugam, K.T. |
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Zeitschrift: | Journal of Bacteriology, Jg. 186 (2004-01-15), Heft 2, S. 580-587 |
Veröffentlichung: | 2004 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
DOI: | 10.1128/JB.186.2.580-587.2004 |
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