Comparative analyses of α-synuclein expression levels in rat brain tissues and transfected cells
In: Neuroscience Letters, Jg. 358 (2004-03-25), Heft 2, S. 95-98
academicJournal
Zugriff:
α-Synuclein may have a role in the genesis of Parkinson''s disease (PD) and other neurodegenerative diseases, and overexpression of α-synuclein in endogenously expressing systems and transfected cell lines has been linked to its cytotoxicity. Because there is no definition of what constitutes normal or high expression levels of α-synuclein, the current studies were undertaken. Protein levels of α-synuclein, and its binding partner, the dopamine transporter (DAT), were examined and semi-quantified in different rat brain regions and compared to the amounts of α-synuclein expressed in Ltk− cells after transfection with known amounts of DNA. Of the regions tested, α-synuclein expression was lowest in dopamine-producing brain areas, substantia nigra and ventral tegmental area; conversely, the expression of DAT was the highest in these tissues. Areas that do not normally degenerate in PD, such as cerebellum, nucleus accumbens and thalamus, expressed the highest levels of α-synculein and very low DAT. Expression of DAT and α-synuclein to levels similar to those observed in rat substantia nigra were obtained after transfection of Ltk− cells with 1–2 μg each of α-synuclein and DAT DNAs, indicating that such transfection conditions produce normal expression levels of these proteins. [Copyright &y& Elsevier]
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Comparative analyses of α-synuclein expression levels in rat brain tissues and transfected cells
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Autor/in / Beteiligte Person: | Wersinger, Christophe ; Banta, Melanie ; Sidhu, Anita |
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Zeitschrift: | Neuroscience Letters, Jg. 358 (2004-03-25), Heft 2, S. 95-98 |
Veröffentlichung: | 2004 |
Medientyp: | academicJournal |
ISSN: | 0304-3940 (print) |
DOI: | 10.1016/j.neulet.2003.12.118 |
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