Improved Aromatic Substitution-Rearrangement-Based Ratiometric Fluorescent Cysteine-Specific Probe and Its Application of Real-Time Imaging under Oxidative Stress in Living Zebrafish.
In: Analytical Chemistry, Jg. 89 (2017-09-05), Heft 17, S. 9567-9573
academicJournal
Zugriff:
Biothiols, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play a crucial role in many physiological processes. Cys production and metabolism is closely connected with Hcy and GSH; meanwhile, the dynamic antioxidant defenses network by Cys is independent of the GSH system, and Cys can serve as a more effective biomarker of oxidative stress. Hence, it is significant and urgent to develop an efficient method for specific detection of Cys over other biothiols (Hcy/GSH). However, most of the present Cys-specific fluorescent probes distinguished Cys from Hcy through response time, which would suffer from an unavoidable interference from Hcy in long-time detection. In this work, in order to improve the selectivity, we employed an improved aromatic substitution-rearrangement strategy to develop a ratiometric Cys-specific fluorescent probe (Cou-SBD-Cl) based on a new fluorescence resonance energy transfer (FRET) coumarin-sulfonyl benzoxadiazole (Cou-SBD) platform for discrimination of Hcy and GSH. Response of Cou-SBD-Cl to Cys would switch FRET on and generate a new yellow fluorescence emission with a 56.1-fold enhancement of ratio signal and a 99 nm emission shift. The desirable dual-color ratiometric imaging was achieved in living cells and normal zebrafish. In addition, probe Cou-SBD-Cl was also applied to real-time monitor Cys fluctuation in lipopolysaccharide-mediated oxidative stress in zebrafish. [ABSTRACT FROM AUTHOR]
Titel: |
Improved Aromatic Substitution-Rearrangement-Based Ratiometric Fluorescent Cysteine-Specific Probe and Its Application of Real-Time Imaging under Oxidative Stress in Living Zebrafish.
|
---|---|
Autor/in / Beteiligte Person: | He, Longwei ; Yang, Xueling ; Xu, Kaixin ; Lin, Weiying |
Link: | |
Zeitschrift: | Analytical Chemistry, Jg. 89 (2017-09-05), Heft 17, S. 9567-9573 |
Veröffentlichung: | 2017 |
Medientyp: | academicJournal |
ISSN: | 0003-2700 (print) |
DOI: | 10.1021/acs.analchem.7b02649 |
Schlagwort: |
|
Sonstiges: |
|