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Higher sequence diversity in the vaginal tract than in blood at early HIV-1 infection.
In: PLoS Pathogens, Jg. 14 (2018-01-18), Heft 1, S. 1-24
Online
academicJournal
Zugriff:
In the majority of cases, human immunodeficiency virus type 1 (HIV-1) infection is transmitted through sexual intercourse. A single founder virus in the blood of the newly infected donor emerges from a genetic bottleneck, while in rarer instances multiple viruses are responsible for systemic infection. We sought to characterize the sequence diversity at early infection, between two distinct anatomical sites; the female reproductive tract vs. systemic compartment. We recruited 72 women from Uganda and Zimbabwe within seven months of HIV-1 infection. Using next generation deep sequencing, we analyzed the total genetic diversity within the C2-V3-C3 envelope region of HIV-1 isolated from the female genital tract at early infection and compared this to the diversity of HIV-1 in plasma. We then compared intra-patient viral diversity in matched cervical and blood samples with three or seven months post infection. Genetic analysis of the C2-V3-C3 region of HIV-1 env revealed that early HIV-1 isolates within blood displayed a more homogeneous genotype (mean 1.67 clones, range 1–5 clones) than clones in the female genital tract (mean 5.7 clones, range 3–10 clones) (p<0.0001). The higher env diversity observed within the genital tract compared to plasma was independent of HIV-1 subtype (A, C and D). Our analysis of early mucosal infections in women revealed high HIV-1 diversity in the vaginal tract but few transmitted clones in the blood. These novel in vivo finding suggest a possible mucosal sieve effect, leading to the establishment of a homogenous systemic infection. [ABSTRACT FROM AUTHOR]
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Higher sequence diversity in the vaginal tract than in blood at early HIV-1 infection.
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Autor/in / Beteiligte Person: | Klein, Katja ; Nickel, Gabrielle ; Nankya, Immaculate ; Kyeyune, Fred ; Demers, Korey ; Ndashimye, Emmanuel ; Kwok, Cynthia ; Chen, Pai-Lien ; Rwambuya, Sandra ; Poon, Art ; Munjoma, Marshall ; Chipato, Tsungai ; Byamugisha, Josaphat ; Mugyenyi, Peter ; Salata, Robert A. ; Morrison, Charles S. ; Arts, Eric J. |
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Zeitschrift: | PLoS Pathogens, Jg. 14 (2018-01-18), Heft 1, S. 1-24 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 1553-7366 (print) |
DOI: | 10.1371/journal.ppat.1006754 |
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