Middle East Respiratory Syndrome Coronavirus Spike Protein Is Not Activated Directly by Cellular Furin during Viral Entry into Target Cells.
In: Journal of Virology, Jg. 92 (2018-10-01), Heft 19, S. 1-12
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Zugriff:
Middle East respiratory syndrome coronavirus (MERS-CoV) utilizes host cellular proteases to enter cells. A previous report shows that furin, which is distributed mainly in the Golgi apparatus and cycled to the cell surface and endosomes, proteolytically activates the MERS-CoV spike (S) protein following receptor binding to mediate fusion between the viral and cellular membranes. In this study, we reexamined furin usage by MERS-CoV using a real-time PCR-based virus cell entry assay after inhibition of cellular proteases. We found that the furin inhibitor dec-RVKR-CMK blocked entry of MERS-CoV harboring an S protein lacking furin cleavage sites; it even blocked entry into furin-deficient LoVo cells. In addition, dec-RVKR-CMK inhibited not only the enzymatic activity of furin but also those of cathepsin L, cathepsin B, trypsin, papain, and TMPRSS2. Furthermore, a virus cell entry assay and a cell-cell fusion assay provided no evidence that the S protein was activated by exogenous furin. Therefore, we conclude that furin does not play a role in entry of MERS-CoV into cells and that the inhibitory effect of dec-RVKR-CMK is specific for TMPRSS2 and cathepsin L rather than furin. [ABSTRACT FROM AUTHOR]
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Middle East Respiratory Syndrome Coronavirus Spike Protein Is Not Activated Directly by Cellular Furin during Viral Entry into Target Cells.
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Autor/in / Beteiligte Person: | Matsuyama, Shutoku ; Shirato, Kazuya ; Kawase, Miyuki ; Terada, Yutaka ; Kawachi, Kengo ; Fukushi, Shuetsu ; Kamitani, Wataru |
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Zeitschrift: | Journal of Virology, Jg. 92 (2018-10-01), Heft 19, S. 1-12 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 0022-538X (print) |
DOI: | 10.1128/JVI.00683-18 |
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