Volltext verfügbar nach Anmeldung bzw. im Campus-Netz.
Reactivation of latent HIV-1 in vitro using an ethanolic extract from Euphorbia umbellata (Euphorbiaceae) latex.
In: PLoS ONE, Jg. 13 (2018-11-27), Heft 11, S. 1-20
Online
academicJournal
Zugriff:
Euphorbia umbellata (E. umbellata) belongs to Euphorbiaceae family, popularly known as Janauba, and its latex contains a combination of phorbol esters with biological activities described to different cellular protein kinase C (PKC) isoforms. Here, we identified deoxi-phorbol esters present in E. umbellata latex alcoholic extract that are able to increase HIV transcription and reactivate virus from latency models. This activity is probably mediated by NF-kB activation followed by nuclear translocation and binding to the HIV LTR promoter. In addition, E. umbellata latex extract induced the production of pro inflammatory cytokines in vitro in human PBMC cultures. This latex extract also activates latent virus in human PBMCs isolated from HIV positive patients as well as latent SIV in non-human primate primary CD4+ T lymphocytes. Together, these results indicate that the phorbol esters present in E. umbellata latex are promising candidate compounds for future clinical trials for shock and kill therapies to promote HIV cure and eradication. [ABSTRACT FROM AUTHOR]
Titel: |
Reactivation of latent HIV-1 in vitro using an ethanolic extract from Euphorbia umbellata (Euphorbiaceae) latex.
|
---|---|
Autor/in / Beteiligte Person: | Valadão, Ana Luiza Chaves ; Pezzuto, Paula ; Silva, Viviane A. Oliveira ; Gonçalves, Barbara Simonson ; Rossi, Átila Duque ; da Cunha, Rodrigo Delvecchio ; Siani, Antonio Carlos ; Tostes, João Batista de Freitas ; Trovó, Marcelo ; Damasco, Paulo ; Gonçalves, Gabriel ; Reis, Rui Manuel ; Aguiar, Renato Santana ; Bento, Cleonice Alves de Melo ; Tanuri, Amilcar |
Link: | |
Zeitschrift: | PLoS ONE, Jg. 13 (2018-11-27), Heft 11, S. 1-20 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (print) |
DOI: | 10.1371/journal.pone.0207664 |
Schlagwort: |
|
Sonstiges: |
|