Presence of a very small population of Thy-1<superscript>+</superscript> , L3T4<superscript>+</superscript> cells producing large amounts of IL-3 in young athymic nude mice.
In: Immunology, Jg. 68 (1989-12-01), Heft 4, S. 557-563
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Zugriff:
A mixture of phorbol myristate acetate (PMA) and ionomycin was found to stimulate spleen and lymph node cells (LNC) from 6 to 8 week-old-athymic BALB/c nude mice, as well as from control +/+ mice, to secrete interleukin-3 (IL-3) in vitro. The specificity of the I L-3 bioassay was attested to by the use of rabbit anti-IL-3 antibodies, and by the detection of an accumulation of IL-3 mRNA. Cytotoxic treatment with relevant antibodies showed that the cells responsible for the IL-3 production in athymic nude mice was Thy-1+, L3T4+, Ly 2-, while both L3T4+ and Ly 2+ cells produced IL-3 in control +/+ mice. Although the levels of IL-3 secreted by nude LNC varied among experiments, nude LNC were able to produce IL-3 at a level comparable to or higher than +/+ LNC. In addition, nude LNC consistently secreted two to three times more granulocyte-macrophage colony-stimulating factor (GM-CSF) than +/+ LNC, and the majority of GM-CSF secretion was dependent on the presence of L3T4+ cells. In contrast, IL-2 production by nude LNC was markedly limited. Since the flow microfluorometry analysis failed to demonstrate the presence of L3T4+ cells (<1%) in nude LNC, compared with 40–50% L3T4+ cells in +/+ LNC, our results suggest that athymic nude mice have a small population of Thy-1+, L3T4+ cells characterized by its ability to secrete IL-3 and GM-CSF at a very high rate. [ABSTRACT FROM AUTHOR]
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Presence of a very small population of Thy-1<superscript>+</superscript> , L3T4<superscript>+</superscript> cells producing large amounts of IL-3 in young athymic nude mice.
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Autor/in / Beteiligte Person: | Kimoto, M. ; de Kossodo, S. ; Kindler, V. ; Detraz, M. ; Vassalli, P. ; Izui, S. |
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Zeitschrift: | Immunology, Jg. 68 (1989-12-01), Heft 4, S. 557-563 |
Veröffentlichung: | 1989 |
Medientyp: | academicJournal |
ISSN: | 0019-2805 (print) |
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