Silencing Y-box binding protein-1 inhibits triple-negative breast cancer cell invasiveness via regulation of MMP1 and beta-catenin expression.
In: Cancer Letters, Jg. 452 (2019-06-28), S. 119-131
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Zugriff:
Y-box binding protein-1 (YB-1), an important transcription and translation regulator protein, is known to increase cancer cell invasiveness and spreading. Here, we report its role in breast cancer, particularly in mediating cell invasion in triple-negative breast cancer (TNBC). YB-1 stable knockdown (shYB-1) significantly reduced the invasive potential of MDA-MB-231 TNBC cells in 2D and 3D (spheroid) cultures. Whole proteome mass spectrometry analysis showed an enrichment of cell adhesion and cell to matrix interaction proteins, notably, matrix metalloproteinase-1 (MMP1) and beta-catenin (CTNNB1), which are known to play critical roles in cancer metastasis. shYB-1 cells exhibited substantial downregulation of MMP1 and CTNNB1 mRNA and protein expression, with reduced MMP1 enzyme activity. YB-1 was also observed to bind to the promoter of MMP1 and overexpression of MMP1 plasmid in shYB-1 cells increased cell invasion. Finally, analysis of tumour samples from the Gene Expression-Based Outcome for Breast Cancer Online (GOBO) database revealed that high gene expressions of YBX1, MMP1 and CTNNB1 predict for a significantly lower 10-year distant metastasis free survival. Altogether, this study shows that YB-1 mediates breast cancer invasion and metastasis via regulation of MMP1 and beta-catenin. [ABSTRACT FROM AUTHOR]
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Silencing Y-box binding protein-1 inhibits triple-negative breast cancer cell invasiveness via regulation of MMP1 and beta-catenin expression.
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Autor/in / Beteiligte Person: | Lim, Jia Pei ; Nair, Sunitha ; Shyamasundar, Sukanya ; Chua, Pei Jou ; Muniasamy, Umamaheswari ; Matsumoto, Ken ; Gunaratne, Jayantha ; Bay, Boon Huat |
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Zeitschrift: | Cancer Letters, Jg. 452 (2019-06-28), S. 119-131 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 0304-3835 (print) |
DOI: | 10.1016/j.canlet.2019.03.014 |
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