High-frequency mutation and recombination are responsible for the emergence of novel porcine reproductive and respiratory syndrome virus in northwest China.
In: Archives of Virology, Jg. 164 (2019-11-01), Heft 11, S. 2725-2733
Online
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Zugriff:
Porcine reproductive and respiratory syndrome (PRRS) is one of the most highly infectious diseases in the pig industry, resulting in enormous economic losses worldwide. In this study, a PRRS virus (PRRSV) strain was isolated from primary porcine alveolar macrophage cells in Xinjiang in northwest China. This new strain was sequenced and designated as XJzx1-2015, and its sequence was then compared to those of other representative PRRSV strains from around the world. Complete genomic characterisation showed that the full-length nucleotide sequence of XJzx1-2015 exhibited low-level similarity to NB/04 (91.6%), JXA1 (90.5%), CH-1a (90.2%), VR-2332 (86.9%), QYYZ (85.7%), and JL580 (82.2%), with the highest similarity to HK13 (91.7%) sequence identity. Nonstructural protein 2 (NSP2) and glycosylated protein (GP) 2 of XJzx1-2015 had deletions of five and two amino acids, respectively, corresponding to strain VR-2332 positions 475-479 and 173-174. Phylogenetic analysis based on complete genome sequences showed that XJzx1-2015 and four other strains from China formed a new subgenotype closely related to other sublineage 8.7 (JXA1-like) strains belonging to the North American genotype. However, phylogenetic analysis based on NSP2 and GP5 showed that XJzx1-2015 clustered with sublineage 8.7 (JXA1-like, CH-1a-like) and lineage 3 (QYYZ-like) strains, respectively. Recombination analysis indicated that XJzx1-2015 is an intersubgenotype recombinant of CH-1a-like and QYYZ-like strains. Overall, our findings demonstrate that XJzx1-2015 is a novel PRRSV strain with a significantly high frequency of mutation and a recombinant between lineage 3 and sublineage 8.7 identified in northwest China. These results provide important insights into PRRSV evolution. [ABSTRACT FROM AUTHOR]
Titel: |
High-frequency mutation and recombination are responsible for the emergence of novel porcine reproductive and respiratory syndrome virus in northwest China.
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Autor/in / Beteiligte Person: | Zhang, Xun ; Li, Yan ; Xiao, Shengzhong ; Yang, Xia ; Chen, Xinkai ; Wu, Peng ; Song, Jiawei ; Ma, Zhenguo ; Cai, Zhuoxuan ; Jiang, Mengmeng ; Zhang, Yanhong ; Yang, Yan ; Zhang, Zhe ; Zhou, Ziheng ; Sheng, Jinliang ; Wang, Heng |
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Zeitschrift: | Archives of Virology, Jg. 164 (2019-11-01), Heft 11, S. 2725-2733 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 0304-8608 (print) |
DOI: | 10.1007/s00705-019-04373-z |
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