Simultaneous Determination of Four Ingredients in Guanxin Danshen Tablets by RP-HPLC.
In: Medicinal Plant, Jg. 10 (2019-10-01), Heft 5, S. 18-20
Online
academicJournal
Zugriff:
[Objectives] This study aimed to establish a method for simultaneous determination of four ingredients in Guanxin Danshen tablets by RP-HPLC. [Methods] HPLC chromatography was adopted with column of Thermo Hypersil-Keystone CJS column (4.6 mm x 250 mm, 5 μm), mobile phase of acetonitrile (A) -0.03 mol/L ammonium acetate (pH adjusted to 2.4 with formic acid) (B), gradient elution (0 -5 min, 5% A; 5-10 min, 5% A→19% A; 10 -40 min, 19% A; 40 -68 min, 19% A→36% A; 68 -90 min, 36% A→95% A), flow rate of 1.0 mL/min and column temperature of 25 °C. [Results] The content of salvianic acid A sodium, protocatechuic aldehyde, salvianolic acid B and tanshinone IIA showed good linear relationship with chromatographic peak area in the range of 3. 310 -18. 66, 0. 039 50 - 0.237 0, 0.750 0 - 4.500, 0. 059 20 - 0. 355 0 peg, respectively. The recovery rate (n = 6) was 101.75%, 96. 86%, 104. 15% and 99.03%, respectively, and the USD was 1.52%, 2.81%, 1.80%, and 1.37% respectively. The established method has good precision, reproducibility and stability. [ Conclusions] This method can be used for the quality control of multiple ingredients of Guanxin Danshen tablets. [ABSTRACT FROM AUTHOR]
Titel: |
Simultaneous Determination of Four Ingredients in Guanxin Danshen Tablets by RP-HPLC.
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Autor/in / Beteiligte Person: | Yougen, WU ; Guihua, ZENG ; Daoan, XIAO ; Yujun, WU ; Yanjun, CHEN ; Juan, LIU |
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Zeitschrift: | Medicinal Plant, Jg. 10 (2019-10-01), Heft 5, S. 18-20 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 2152-3924 (print) |
DOI: | 10.19600/j.cnki.issn2152-3924.2019.05.005 |
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