Carbapenem inactivation method using bacterial lysate and MOPS (LCIM): a very sensitive method for detecting carbapenemase-producing Acinetobacter species.
In: Journal of Antimicrobial Chemotherapy (JAC), Jg. 75 (2020-10-01), Heft 10, S. 2812-2816
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Zugriff:
Objectives: Detection of carbapenem-hydrolysing class D β-lactamase (CHDL)-producing Acinetobacter spp. is critical for understanding antibiotic resistance. In this study, we compared the available detection techniques derived from the carbapenem inactivation method (CIM), using CHDL-producing Acinetobacter spp., and developed a modified method that uses bacterial lysate (lysate CIM; LCIM). Methods: A total of 159 Acinetobacter spp. (102 carbapenemase producers and 57 non-producers) and 14 Pseudomonas spp. (7 carbapenemase producers and 7 non-producers) were tested. Modified CIM, simplified CIM, CIMTris, Triton-CIM and LCIM were compared using these strains. Distinct from the CIM, LCIM includes a longer incubation period (4 h) with 2.0% Triton X-100 (v/v) in 20 mM MOPS buffer instead of water. Results: The sensitivity/specificity of the modified CIM, simplified CIM, CIMTris, Triton-CIM and LCIM were 71.6%/100%, 66.1%/89.1%, 88.1%/95.3%, 80.7%/100% and 97.2%/100%, respectively. LCIM was the most sensitive and specific. Conclusions: Use of bacterial lysate and MOPS increased the sensitivity of the CIM in detecting CHDL-producing Acinetobacter spp. [ABSTRACT FROM AUTHOR]
Titel: |
Carbapenem inactivation method using bacterial lysate and MOPS (LCIM): a very sensitive method for detecting carbapenemase-producing Acinetobacter species.
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Autor/in / Beteiligte Person: | Yamada, Kageto ; Aoki, Kotaro ; Nagasawa, Tatsuya ; Imai, Waka ; Sasaki, Masakazu ; Murakami, Hinako ; Morita, Toshisuke ; Ishii, Yoshikazu ; Tateda, Kazuhiro |
Zeitschrift: | Journal of Antimicrobial Chemotherapy (JAC), Jg. 75 (2020-10-01), Heft 10, S. 2812-2816 |
Veröffentlichung: | 2020 |
Medientyp: | academicJournal |
ISSN: | 0305-7453 (print) |
DOI: | 10.1093/jac/dkaa238 |
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