A Stronger Transcription Regulatory Circuit of HIV-1C Drives the Rapid Establishment of Latency with Implications for the Direct Involvement of Tat.
In: Journal of Virology, Jg. 94 (2020-10-01), Heft 19, S. 1-36
Online
academicJournal
Zugriff:
The magnitude of transcription factor binding site variation emerging in HIV-1 subtype C (HIV-1C), especially the addition of NF-μB motifs by sequence duplication, makes the examination of transcriptional silence challenging. How can HIV-1 establish and maintain latency despite having a strong long terminal repeat (LTR)? We constructed panels of subgenomic reporter viral vectors with varying copy numbers of NF-μB motifs (0 to 4 copies) and examined the profile of latency establishment in Jurkat cells. Surprisingly, we found that the stronger the viral promoter, the faster the latency establishment. Importantly, at the time of commitment to latency and subsequent points, Tat levels in the cell were not limiting. Using highly sensitive strategies, we demonstrate the presence of Tat in the latent cell, recruited to the latent LTR. Our data allude, for the first time, to Tat establishing a negative feedback loop during the late phases of viral infection, leading to the rapid silencing of the viral promoter. [ABSTRACT FROM AUTHOR]
Titel: |
A Stronger Transcription Regulatory Circuit of HIV-1C Drives the Rapid Establishment of Latency with Implications for the Direct Involvement of Tat.
|
---|---|
Autor/in / Beteiligte Person: | Chakraborty, Sutanuka ; Kabi, Manisha ; Ranga, Udaykumar |
Link: | |
Zeitschrift: | Journal of Virology, Jg. 94 (2020-10-01), Heft 19, S. 1-36 |
Veröffentlichung: | 2020 |
Medientyp: | academicJournal |
ISSN: | 0022-538X (print) |
DOI: | 10.1128/jvi.00503-20 |
Schlagwort: |
|
Sonstiges: |
|