CRYOPRESERVATION OF IN VITRO-PRODUCED BOVINE EMBRYOS BY VITRIFICATION: IN PURSUIT OF A SIMPLIFIED, STANDARDIZED PROCEDURE THAT IMPROVES PREGNANCY RATES TO PROMOTE CATTLE INDUSTRY USE.
In: Biotechnology in Animal Husbandry, Jg. 36 (2020-08-01), Heft 3, S. 251-270
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Zugriff:
The goal of cryopreservation is to retain the original stage of gametes and embryos after they have endured cooling and warming. Slow freezing is a standard method for in vivo-derived bovine embryo cryopreservation, threefifths of such embryos being frozen by this method globally. However, it is evident that slow freezing is not efficient for cryopreserving in vitro-produced bovine embryos. Hence, only one-third of in vitro-produced bovine embryos are cryopreserved. Vitrification is a preferred method for storage of human embryos; consequently, it has been explored as a novel means to store in vitro-produced bovine embryos, for which it shows considerable promise as an alternative to slow freezing. This is due to several reasons: vitrification is often less time-consuming than slow freezing; it does not need expensive slow rate freezing machines; and it has been proven to have comparatively higher survival rates. Yet, in the cattle industry vitrification continues to present shortcomings, such as possible toxicity of vitrification solutions and failure to standardize methods, which pose a challenge for its application to in vitro-produced bovine embryos. Therefore, determining the most suitable procedure is crucial to make vitrification more practical in commercial settings. [ABSTRACT FROM AUTHOR]
Cilj krioprezervacije je zadržati prvobitnu fazu polnih ćelija i embriona nakon što izdrže hlađenje i zagrevanje. Polako zamrzavanje je standardni metod za krioprezervaciju goveđih embriona in vivo, pri čemu se tri petine takvih embriona globalno zamrzava ovom metodom. Međutim, očigledno je da sporo zamrzavanje nije efikasno za krioprezervaciju goveđih embriona proizvedenih in vitro. Dakle, samo jedna trećina goveđih embriona proizvedenih in vitro je krio-konzervirana. Vitrifikacija je poželjna metoda za skladištenje ljudskih embriona; shodno tome, istražena je kao novo sredstvo za čuvanje in vitro proizvedenih goveđih embriona, za koje se pokazala kao značajna alternativa sporom zamrzavanju, i to iz nekoliko razloga: vitrifikacija često zahteva manje vremena nego sporo zamrzavanje, nisu potrebne skupe mašine za zamrzavanje sa sporim tempom, i dokazano je da ima relativno veće stope preživljavanja. Ipak, u stočarstvu/govedarstvu, vitrifikacija i dalje pokazuje nedostatke, kao što su moguća toksičnost rastvora za vitrifikaciju i neuspeh u standardizaciji metoda, što predstavlja izazov za njegovu primenu na in vitro proizvedene embrione goveda. Zbog toga je određivanje najprikladnijeg postupka presudno kako bi se vitrifikacija učinila praktičnijom u komercijalnim uslovima. [ABSTRACT FROM AUTHOR]
Titel: |
CRYOPRESERVATION OF IN VITRO-PRODUCED BOVINE EMBRYOS BY VITRIFICATION: IN PURSUIT OF A SIMPLIFIED, STANDARDIZED PROCEDURE THAT IMPROVES PREGNANCY RATES TO PROMOTE CATTLE INDUSTRY USE.
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Autor/in / Beteiligte Person: | Van Huong Do ; Taylor-Robinson, Andrew W. |
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Zeitschrift: | Biotechnology in Animal Husbandry, Jg. 36 (2020-08-01), Heft 3, S. 251-270 |
Veröffentlichung: | 2020 |
Medientyp: | academicJournal |
ISSN: | 1450-9156 (print) |
DOI: | 10.2298/BAH2003251H |
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