A comparison of gene expression profiles produced by SAGE, long SAGE, and oligonucleotide chips
In: Genomics, Jg. 84 (2004-10-01), Heft 4, S. 631-636
academicJournal
Zugriff:
A comparison study of short SAGE versus GeneChip and long SAGE was conducted to determine if data were interchangeable between the techniques. Although SAGE and Affymetrix chip expression levels showed a significant correlation using the set of genes for which there was reliable and unambiguous mapping from tag-to-gene identification, correlation coefficients were low (0.51 for the Spearman''s correlation coefficient with p < 0.0001). A quantitative analysis comparing the use of 10-bp SAGE tags to 17-bp SAGE tags indicated that the short SAGE technology was more efficient at identifying differentially expressed tags. However, 4–7% of the 10-bp tags were redundancies that could have been resolved by long SAGE and 10–20% of the short SAGE tags had no obvious match to currently annotated human transcript sequences. [Copyright &y& Elsevier]
| Titel: |
A comparison of gene expression profiles produced by SAGE, long SAGE, and oligonucleotide chips
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| Autor/in / Beteiligte Person: | Lu, Jun ; Lal, Anita ; Merriman, Barry ; Nelson, Stan ; Riggins, Gregory |
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| Zeitschrift: | Genomics, Jg. 84 (2004-10-01), Heft 4, S. 631-636 |
| Veröffentlichung: | 2004 |
| Medientyp: | academicJournal |
| ISSN: | 0888-7543 (print) |
| DOI: | 10.1016/j.ygeno.2004.06.014 |
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