NPF activates a specific NPF receptor and regulates food intake in Pacific abalone Haliotis discus hannai.
In: Scientific Reports, Jg. 11 (2021-10-22), Heft 1, S. 1-16
Online
academicJournal
Zugriff:
Neuropeptides function through G protein-coupled receptors (GPCRs) with high specificity, implying a significant degree of neuropeptide-GPCR coevolution. However, potential neuropeptide signaling systems in non-chordates are relatively elusive. We determined the specificity of the neuropeptide F (Hdh-NPF) signaling system with a cognate receptor (Hdh-NPFR) in the Pacific abalone, Haliotis discus hannai. Phylogenetic and exon–intron arrangement analyses of bilaterian NPF and the chordate ortholog NPY with their receptor sequences revealed a likely common ancestor, and Hdh-NPFR was similar to the NPYR2 subtype among the NPYR1, NPYR2, and NPYR5 subtypes. Among four Hdh-NPFR-related receptors, Hdh-NPFR specifically responded to Hdh-NPF peptide, supported by the dose–response luciferase reporter curve, intracellular Ca2+ mobilization, and phosphorylation of ERK1/2 and its inhibition with a protein kinase C inhibitor. Peptide fragmentations and shuffling of Hdh-NPF with human NPY could not activate the cellular response of Hdh-NPFR. Three-dimensional in silico modeling suggested that interaction of Hdh-NPF C-terminal amino acids with the extracellular loops of Hdh-NPFR is critical for Hdh-NPFR activation. In vivo injection of Hdh-NPF peptide increased food consumption, and knockdown of Hdh-NPF expression decreased food consumption in Pacific abalone. These findings provide evidence for co-evolution of the NPF/Y ligand-receptor system, enabling further research on mollusk orexigenic neuropeptides. [ABSTRACT FROM AUTHOR]
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NPF activates a specific NPF receptor and regulates food intake in Pacific abalone Haliotis discus hannai.
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Autor/in / Beteiligte Person: | Kim, Kyeong Seop ; Kim, Mi Ae ; Park, Keunwan ; Sohn, Young Chang |
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Zeitschrift: | Scientific Reports, Jg. 11 (2021-10-22), Heft 1, S. 1-16 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-021-00238-1 |
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