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Generation of a tendon-like tissue from human iPS cells.
In: Journal of Tissue Engineering, Jg. 13 (2022-01-21), S. 1-13
Online
academicJournal
Zugriff:
Tendons and ligaments are essential connective tissues that connect the muscle and bone. Their recovery from injuries is known to be poor, highlighting the crucial need for an effective therapy. A few reports have described the development of artificial ligaments with sufficient strength from human cells. In this study, we successfully generated a tendon-like tissue (bio-tendon) using human induced pluripotent stem cells (iPSCs). We first differentiated human iPSCs into mesenchymal stem cells (iPSC-MSCs) and transfected them with Mohawk (Mkx) to obtain Mkx-iPSC-MSCs, which were applied to a newly designed chamber with a mechanical stretch incubation system. The embedded Mkx-iPSC-MSCs created bio-tendons and exhibited an aligned extracellular matrix structure. Transplantation of the bio-tendons into a mouse Achilles tendon rupture model showed host-derived cell infiltration with improved histological score and biomechanical properties. Taken together, the bio-tendon generated in this study has potential clinical applications for tendon/ligament-related injuries and diseases. [ABSTRACT FROM AUTHOR]
| Titel: |
Generation of a tendon-like tissue from human iPS cells.
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| Autor/in / Beteiligte Person: | Tsutsumi, Hiroki ; Kurimoto, Ryota ; Nakamichi, Ryo ; Chiba, Tomoki ; Matsushima, Takahide ; Fujii, Yuta ; Sanada, Risa ; Kato, Tomomi ; Shishido, Kana ; Sakamaki, Yuriko ; Kimura, Tsuyoshi ; Kishida, Akio ; Asahara, Hiroshi |
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| Zeitschrift: | Journal of Tissue Engineering, Jg. 13 (2022-01-21), S. 1-13 |
| Veröffentlichung: | 2022 |
| Medientyp: | academicJournal |
| ISSN: | 2041-7314 (print) |
| DOI: | 10.1177/20417314221074018 |
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