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Lysophosphatidylinositol Induced Morphological Changes and Stress Fiber Formation through the GPR55-RhoA-ROCK Pathway.
In: International Journal of Molecular Sciences, Jg. 23 (2022-09-15), Heft 18, S. N.PAG- (13S.)
Online
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Zugriff:
We previously reported that lysophosphatidylinositol (LPI) functions as an endogenous agonist of GPR55, a novel cannabinoid receptor. However, the physiological roles of LPI-GPR55 have not yet been elucidated in detail. In the present study, we found that LPI induced morphological changes in GPR55-expressing HEK293 cells. LPI induced the cell rounding of GPR55-expressing HEK293 cells but not of empty-vector-transfected cells. LPI also induced the activation of small GTP-binding protein RhoA and increased stress fiber formation in GPR55-expressing HEK293 cells. The inhibition of RhoA and Rho kinase ROCK by the C3 exoenzyme and the ROCK inhibitor reduced LPI-induced cell rounding and stress fiber formation. These results clearly indicated that the LPI-induced morphological changes and the assembly of the cytoskeletons were mediated through the GPR55-RhoA-ROCK pathway. [ABSTRACT FROM AUTHOR]
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Lysophosphatidylinositol Induced Morphological Changes and Stress Fiber Formation through the GPR55-RhoA-ROCK Pathway.
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Autor/in / Beteiligte Person: | Nakajima, Keisuke ; Oka, Saori ; Tanikawa, Takashi ; Nemoto-Sasaki, Yoko ; Matsumoto, Naoki ; Ishiguro, Hiroki ; Arata, Yoichiro ; Sugiura, Takayuki ; Yamashita, Atsushi |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 23 (2022-09-15), Heft 18, S. N.PAG- (13S.) |
Veröffentlichung: | 2022 |
Medientyp: | academicJournal |
ISSN: | 1661-6596 (print) |
DOI: | 10.3390/ijms231810932 |
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