Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine.
In: Journal of Virological Methods, Jg. 314 (2023-04-01), S. N.PAG
academicJournal
Zugriff:
Maternal acute primary cytomegalovirus (CMV) infection during the first trimester may cause severe long-term sequelae in newborns. For risk assessment, serological screening is routinely performed in pregnant women based on IgM, IgG and avidity tests using whole-virus antigen. A recent study evaluated the diagnostic value of recombinant protein-based ELISAs as second-line tests in pregnancy CMV screening, including anti-p52 IgM and anti-gB IgG as markers defining the early and late phase of infection, respectively. In the present study, these recombinant ELISAs were used as first-line screening tests in daily laboratory routine and compared to lysate-based assays with respect to [i] the number of conclusive results obtained with the initial sample and [ii] the underlying workload. 553 unselected routine serum samples from pregnant women were tested for anti-CMV IgM and IgG antibodies using lysate-based ELISAs and avidity testing. Anti-CMV IgM antibodies against recombinant p52 and anti-CMV IgG antibodies against recombinant glycoprotein B (gB) were also determined by ELISA. All assays were performed and interpreted according to the manufacturer's instructions. For lysate-based IgM, IgG and avidity testing, 84.6 % of samples yielded conclusive results in a total of 1156 tests, while 15.4 % needed follow-up testing of a consecutive sample. Anti-p52 CMV IgM and anti-gB CMV IgG testing produced conclusive results for 92.8 % of samples in a total of 1026 tests, while 7.2 % samples required follow-up testing. The first-line use of ELISAs measuring anti-p52 CMV IgM and anti-gB CMV IgG antibodies to test for maternal CMV infection increases the number of conclusive results derived from an initial serum sample while requiring a considerably lower number of tests compared to the lysate-based approach. For day-to-day routines in a diagnostic laboratory, this high efficiency of the recombinant testing approach has significant practical relevance. • Screening for maternal CMV infection is important for risk assessment in newborns. • Routine testing is performed using immunoassays based on whole-virus antigens. • Alternatively, serodiagnosis is performed using assays with recombinant antigens. • Recombinant testing requires fewer test on consecutive samples. • The high efficiency of recombinant testing is important for diagnostic laboratories. [ABSTRACT FROM AUTHOR]
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Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine.
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Autor/in / Beteiligte Person: | Müller, Jens ; Flindt, Juliane ; Pollmann, Marc ; Saschenbrecker, Sandra ; Borchardt-Lohölter, Viola ; Warnecke, Jens M. |
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Zeitschrift: | Journal of Virological Methods, Jg. 314 (2023-04-01), S. N.PAG |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 0166-0934 (print) |
DOI: | 10.1016/j.jviromet.2023.114685 |
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