石榴皮多酚对妊娠期糖尿病大鼠胰岛素抵抗及氧化应激损伤的影响.

Objective: To investigate the effects of pomegranate peel polyphenols on insulin resistance (IR) and oxidative stress injury in pregnant diabetes rats. Methods: Five pregnant rats were randomly divided into control group, model group and PPPs group with 15 rats each. GDM model rats were prepared by intraperitoneal injection of 45 mg/kg streptozotocin in model group and PPPs group, and normal saline was injected intraperitoneal in control group. After the model was successfully prepared, rats in the PPPs group were given 300 mg/kg PPPs by gavage, and rats in the control group and the model group were given equal volumes of normal saline by gavage. HbA1c, fasting blood glucose (FBG), fasting insulin (FINS) and tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), IL-6, high sensitivity C-reactive protein (hs-CRP), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), uric acid (UA), blood urea nitrogen (BUN), serum creatinine (SCr) and urine β-N-acetylglucosaminidase (NAG) and kidney damage molecules-1 (KIM-1) in the fasting tail vein blood were detected at 14 days after administration. The homeostasis model of insulin resistance (HOMA-IR) was caculated. Results: Serum HbA1c, FBG, FINS, TNF-α, IL-1β, IL-6, hs-CRP, MDA, UA, BUN, SCR, urine NAG and KIM-1, HOMA-IR of rats in model group and PPPs group were higher than those in the control group, while the activities of SOD and CAT were lower than those in the control group (P<0.05). Serum HbA1c, FBG, FINS, TNF-α, IL-1β, IL-6, hs-CRP, MDA, UA, BUN, SCR, urine NAG and KIM-1, HOMA-IR of rats in PPPs group were lower than those in the model group, while the activities of SOD and CAT were higher than those in the model group (P<0.05). Conclusions: PPPs can significantly reduce insulin resistance and oxidative stress levels in pregnant diabetes rats, and improve blood sugar and kidney damage. [ABSTRACT FROM AUTHOR]

目的：探讨石榴皮多酚 (PPPs) 对妊娠期糖尿病 (GDM) 大鼠胰岛素抵抗 (IR) 及氧化应激损伤的影响。方法：45 只孕鼠随机分 为对照组、模型组和 PPPs 组, 各 15 只。模型组和 PPPs 组孕鼠经腹腔注射 45mg/kg 链脲佐菌素制备 GDM 模型大鼠, 对照组经腹 腔注射生理盐水。模型制备成功后给予 PPPs 组大鼠按 300 mg/kg PPPs 灌胃, 对照组和模型组灌胃等体积生理盐水。给药 14 d 后 检测空腹尾静脉血中糖化血红蛋白 (HbA1c) 、空腹血糖 (FBG) 、空腹胰岛素 (FINS) 、肿瘤坏死因子 -α (TNF-α) 、白细胞介素 -1β (IL-1β) 、IL-6、超氧化物歧化酶 (SOD) 、超敏 C 反应蛋白 (hs-CRP) 、丙二醛 (MDA) 、过氧化氢酶 (CAT) 、尿酸 (UA) 、尿素氮 (BUN) 、血清肌酐 (SCr) 和尿液 β-N- 乙酰氨基葡萄糖苷酶 (NAG) 、肾损伤分子 1 (KIM-1) 水平, 计算稳态模型的胰岛素抵抗指数 (HOMA-IR) 。结果：模型组和 PPPs 组大鼠血清 HbA1c、FBG、FINS、TNF-β、IL-1β、IL-6、hs-CRP、MDA、UA、BUN、SCr 和尿液 NAG、KIM-1 水平以及 HOMA-IR 显著高于对照组, SOD 和 CAT 活性显著低于对照组 (P<0.05) 。PPPs 组大鼠血清 HbA1c、FBG、 FINS、TNF-α、IL-1β、IL-6、hs-CRP、MDA、UA、BUN、SCr 和尿液 NAG、KIM-1 水平以及 HOMA-IR 均显著低于模型组, SOD 和 CAT 活性显著高于对照组 (P<0.05) 。结论：PPPs 能够显著减轻妊娠期糖尿病大鼠胰岛素抵抗、氧化应激水平, 改善血糖和肾损伤。 [ABSTRACT FROM AUTHOR]