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Targeting Melanoma-Associated Fibroblasts (MAFs) with Activated γδ (Vδ2) T Cells: An In Vitro Cytotoxicity Model.
In: International Journal of Molecular Sciences, Jg. 24 (2023-08-15), Heft 16, S. 12893-12909
Online
academicJournal
Zugriff:
The tumor microenvironment (TME) has gained considerable scientific attention by playing a role in immunosuppression and tumorigenesis. Besides tumor cells, TME is composed of various other cell types, including cancer-associated fibroblasts (CAFs or MAFs when referring to melanoma-derived CAFs) and tumor-infiltrating lymphocytes (TILs), a subpopulation of which is labeled as γδ T cells. Since the current anti-cancer therapies using γδ T cells in various cancers have exhibited mixed treatment responses, to better understand the γδ T cell biology in melanoma, our research group aimed to investigate whether activated γδ T cells are capable of killing MAFs. To answer this question, we set up an in vitro platform using freshly isolated Vδ2-type γδ T cells and cultured MAFs that were biobanked from our melanoma patients. This study proved that the addition of zoledronic acid (1–2.5 µM) to the γδ T cells was necessary to drive MAFs into apoptosis. The MAF cytotoxicity of γδ T cells was further enhanced by using the stimulatory clone 20.1 of anti-BTN3A1 antibody but was reduced when anti-TCR γδ or anti-BTN2A1 antibodies were used. Since the administration of zoledronic acid is safe and tolerable in humans, our results provide further data for future clinical studies on the treatment of melanoma. [ABSTRACT FROM AUTHOR]
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Targeting Melanoma-Associated Fibroblasts (MAFs) with Activated γδ (Vδ2) T Cells: An In Vitro Cytotoxicity Model.
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Autor/in / Beteiligte Person: | Hajdara, Anna ; Çakır, Uğur ; Érsek, Barbara ; Silló, Pálma ; Széky, Balázs ; Barna, Gábor ; Faqi, Shaaban ; Gyöngy, Miklós ; Kárpáti, Sarolta ; Németh, Krisztián ; Mayer, Balázs |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 24 (2023-08-15), Heft 16, S. 12893-12909 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 1661-6596 (print) |
DOI: | 10.3390/ijms241612893 |
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