An optimized methodology for the determination of multiclass organic ultraviolet sunscreens and metabolites in human milk through chromatographic and chemometric resolution.
In: Talanta, Jg. 270 (2024-04-01), S. N.PAG
academicJournal
Zugriff:
Organic UV filters (UVFS) are used to mitigate the dermal effects associated with health risks from UV radiation, making them essential in personal care products. UVFS are frequently identified in environmental samples due to their high lipophilicity and persistence, underscoring the urgency of comprehensive assessments and regulatory measures aimed at safeguarding ecosystems and human health. The present study reports a multiclass analytical method for determining 16 UV sunscreens and metabolites in breast milk based on an ultrasound-assisted-dispersive liquid-liquid micro-extraction (UA-DLLME) with further chromatographic and chemometric resolution. The experimental conditions of the UA-DLLME were optimized through the implementation of the Design of Experiment tools. To model the responses, least-squares and artificial neural network methodologies were implemented. The optimal conditions were found by employing the desirability function. The samples were analyzed through reverse-phase liquid chromatographic separation, UV diode array, and fast-scanning fluorescence detection. The chromatographic analysis enabled the resolution of 16 analytes in a total time of 13.0 min. Multivariate curve resolution-alternating least-square (MCR-ALS) modelling was implemented to resolve analytes that were not fully resolved and to determine analytes that coeluted with endogenous components of the breast milk samples. An enrichment factor of 5-fold concentration was obtained with this methodology, reaching recoveries between 65 % and 105 % for 13 multiclass UV sunscreens and metabolites in breast milk samples with RSD % and REP % lower than 12 %. [Display omitted] • An optimized UA-DLLME strategy to determine sunscreens and metabolites in breast milk. • Chromatography with dual detection to ensure selectivity and sensitivity. • RSM and ANN-based modelling for UA-DLLME optimization. • MCR-ALS chemometric modelling to solve overlapped analytes and discriminate endogenous compound signals. • Validation assays assert the reliability and precision of the developed method. [ABSTRACT FROM AUTHOR]
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An optimized methodology for the determination of multiclass organic ultraviolet sunscreens and metabolites in human milk through chromatographic and chemometric resolution.
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Autor/in / Beteiligte Person: | Alcaraz, Mirta R. ; Espinosa-Mansilla, Anunciación ; Durán-Merás, Isabel ; Muñoz de la Peña, Arsenio |
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Zeitschrift: | Talanta, Jg. 270 (2024-04-01), S. N.PAG |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 0039-9140 (print) |
DOI: | 10.1016/j.talanta.2023.125560 |
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