Catalytic hairpin assembly triggering amplified DNAzyme-feedback for sensitive detection of hepatitis C virus genotype 1b.
In: Talanta, Jg. 271 (2024-05-01), S. N.PAG
academicJournal
Zugriff:
Developing a simple, reliable, and sensitive hepatitis C virus (HCV) genetic sensing platform is of great significance for diagnosing diseases and selecting appropriate antiviral treatments. Herein, a tandem nucleic acid amplification strategy for sensitive detection of HCV genotype 1b (HCV-1b) was developed by stringing the catalytic hairpin assembly (CHA) and the triggered DNAzyme amplifier. The hairpin reactants were initiated by the target to produce lots of triggering double-stranded DNA sequences which can efficiently activate the subsequent blocked DNAzyme. Thereby, the continuous cleavage of substrate was realized, resulting in the fluorescence signal amplification. The DNA-based isothermal CHA-DNAzyme (CDz) sensing platform was successfully applied for sensitive detection of HCV-1b with the limit of detection (84 pM) and showed good selectivity. Moreover, the practical detection of target DNA in the complex biologic matrix indicated that the developing strategy had good potential for early HCV infection diagnosis. A tandem nucleic acid amplification strategy for sensitive detection of HCV genotype 1b (HCV-1b) was developed by connecting the catalytic hairpin assembly (CHA) and the triggered DNAzyme fluorescence amplifiers. [Display omitted] • An isothermal concatenated nucleic acid amplification system consists of CHA and DNAzyme amplifiers • The multiple successive amplification cascades are applied to the sensitive detection of HCV-1b • The sensing system shows good selectivity to distinguish single-base mismatched target • This versatile platform has great potential for clinical diagnosis and prognosis [ABSTRACT FROM AUTHOR]
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Catalytic hairpin assembly triggering amplified DNAzyme-feedback for sensitive detection of hepatitis C virus genotype 1b.
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Autor/in / Beteiligte Person: | Xu, Yuting ; Li, Lili ; Yang, Yumeng ; Huang, Chengzhi ; Zou, Hongyan |
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Zeitschrift: | Talanta, Jg. 271 (2024-05-01), S. N.PAG |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 0039-9140 (print) |
DOI: | 10.1016/j.talanta.2024.125754 |
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