柳芽粗多糖超声辅助提取工艺优化及抗氧化活性分析.

In order to optimize the extraction process of crude polysaccharide of willow bud (CPW, Crude polysaccharide of willow bud) and to investigate its in vitro antioxidant activity, the experiment was carried out to extract crude polysaccharides from willow buds by ultrasound-assisted hydroalcohol precipitation and optimized using response surface analysis; the in vitro antioxidant effect of CPW was evaluated by detecting its total antioxidant capacity, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals (DPPH·) as well as its ability to inhibit superoxide anion radicals (O2 - ·) and hydroxyl radicals (·OH), using vitamin C (vitamin C, VC) as a control. The results showed that the optimal extraction process for CPW was 70∶1 (mL/g) liquid/feed ratio, 56 ℃ water bath temperature, and 440 W ultrasonic power. The extraction process was used to achieve 6.6% extraction of CPW. In addition, different concentrations of CPW were effective in scavenging DPPH radicals and inhibiting the production of ·OH and O2 - ·. It shows that the process is practicable and the extracted CPW has better antioxidant capacity [ABSTRACT FROM AUTHOR]

为了优化柳芽粗多糖（crude polysaccharide of willow bud，CPW）提取工艺并考察其体外 抗氧化活性，试验采用超声辅助水提醇沉法提取柳芽粗多糖，并运用响应面分析法进行优化；以维生素 C（vitamin C，VC）为对照，通过比色法检测其总抗氧化能力、清除1,1-二苯基-2-三硝基苯肼（1,1-diphe‐ nyl-2-picrylhydrazyl，DPPH）自由基（DPPH·）以及抑制超氧阴离子自由基（O2 - ·）和羟自由基（·OH）的能 力，评价 CPW 体外抗氧化效果。结果表明：CPW 最佳提取工艺为液料比 70∶1（mL/g），水浴温度 56 ℃，超声功率 440 W；采用该提取工艺对 CPW 的提取率达到 6.6%。不同浓度 CPW 能够有效清除 DPPH·和抑制·OH 和 O2 - ·的产生。说明优化的 CPW 超声辅助提取工艺切实可行，且所提取的 CPW 抗氧化能力较好。 [ABSTRACT FROM AUTHOR]