ROLES OF MIR155HG AND TNF-α IN EVALUATION OF PROGNOSIS OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS.

Background: Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease characterized by multi-organ multi-system inflammation, causing severe damage to various organs or systems. Recent studies have shown that miR-155 can affect the progression of Lupus Nephritis via regulating TNF-a. The present study aims to explore the roles of MIR155HG and TNF-a in the evaluation of prognosis of patients with SLE, so as to provide a basis for clinical work. Methods: A total of 130 patients with SLE admitted to our hospital were selected, were selected from June 2015 to December 2017., and the SLE disease activity index (SLEDAI) score was given. 1 he expressions of MIR155HG and 1 NF-a were detected via quantitative reverse transcription-polyme-rase chain reaction (qRT-PCR), the incidence of complications during treatment was observed, and the associations of MIR155HG and TNF-a with SLEDAI before treatment and complications were analyzed. All patients were followed up after discharge, and the related factors to the prognosis of patients were analyzed via Cox regression analysis. Results: The levels of MIR155HG and TNF-a were higher in patients with an SLEDAI score of 10-14 points than those in patients with an SLEDAI score of 5-9 points and 0-4 points. MIR155HG and TNF-a were positively correlated with the incidence of infection, renal damage and cardiac damage (r=0.623, 0.533 and 0.621; r=0.431, 0.498 and 0.552) (P<0.05). Moreover, there was also a positive correlation (r=0.3398, PcO.001) between the expressions of serum MIR155HG and TNF-a in SLE patients. SLEDAI score Ž10 points, complications during hospitalization, and highly-expressed MIR155HG and TNF-a were risk factors related to the prognosis of patients. Conclusions: MIR155HG and TNF-a affect the activity of SLE, and the high expressions of them promote the occurrence of such complications as infection, renal damage and cardiac damage, harming the prognosis. [ABSTRACT FROM AUTHOR]

Uvod: Sistemski eritematozni lupus (SLE) je hronična inflam-atorna autoimuna bolest koju karakteriše višeorganska mul-tisistemska upala, koja izaziva ozbiljna oštećenja različitih organa ili sistema. Nedavne studije su pokazale da miR-155 može uticati na napredovanje lupus nefritisa regulacijom TNF-a. Ova studija ima za cilj da istraži uloge MIR155HG i TNF-a u proceni prognoze pacijenata sa SLE, kako bi se pružila osnova za klinički rad. Metode: Odabrano je ukupno 130 pacijenata sa SLE primljenim u našu bolnicu, odabrano od juna 2015. do decembra 2017. godine i dat je skor indeksa aktivnosti SLE bolesti (SLEDAI). Ekspresije MIR155HG i TNF-a su detektovane kvantitativnom reverznom transkripcijom-polimerazom lančanom reakcijom (qRT-PCR), uočena je učestalost komplikacija tokom lečenja, a analizirane su asocijacije MIR155HG i TNF-a sa SLEDAI pre tretmana i komplikacija. Svi pacijenti su praćeni nakon otpusta, a faktori povezani sa prognozom pacijenata analizirani su Cox regresionom analizom. Rezultati: Nivoi MIR155HG i TNF-a bili su viši kod pacijenata sa SLEDAI skorom od 10-14 poena nego kod pacijenata sa SLEDAI skorom od 5-9 poena i 0-4 poena. MIR155HG i TNF-a su bili u pozitivnoj korelaciji sa inciden-com infekcije, oštećenja bubrega i oštećenja srca (r=0,623, 0,533 i 0,621; r=0,431, 0,498 i 0,552) (P<0,05). Štaviše, postojala je i pozitivna korelacija (r=0,3398, P<0,001) izme u ekspresije serumskog MIR155HG i 1 NF-a kod pacijenata sa SLE. SLEDAI skor >10 poena, komplikacije tokom hospitalizacije i visoko eks pri mi ran i MIR155HG i TNF-a bili su faktori rizika koji se odnose na prognozu pacijenata. Zaključak: MIR155HG i TNF-a utiču na aktivnost SLE, a njihova visoka ekspresija promoviše nastanak takvih komplikacija kao što su infekcija, oštećenje bubrega i srčana oštećenja, što šteti prognozi. [ABSTRACT FROM AUTHOR]