Induction of cross-reactive immune responses to NTS-DBL-1α/x of PfEMP1 and in vivo protection on challenge with Plasmodium falciparum
In: Vaccine, Jg. 24 (2006-08-28), Heft 35/36, S. 6140-6154
academicJournal
Zugriff:
Abstract: The interactions of Plasmodium falciparum infected erythrocytes parasitized red blood cells (pRBC) with endothelial receptors and erythrocytes are mediated by multiple Duffy-binding like (DBL) and cysteine-rich interdomain region (CIDR) domains harboured in the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). The success of a subunit vaccine based on PfEMP1 depends on its ability to elicit cross-reactive responses to a substantial number of PfEMP1 variants. We have here evaluated serological PfEMP1 cross-reactivity by immunizing rats with phylogenetically diverse recombinant NTS-DBL-1α/x fusion domains from the 3D7 genome parasite emulsified in Montanide ISA 720. Cross-reactivity was elicited to these diverse DBL-1α/x domains as measured by ELISA and by immunoblotting. Employing a novel in vivo model of human infected erythrocyte sequestration, immunized animals were challenged with the FCR3S1.2 clone and cross-protection in terms of reduction in lung sequestration amounting to approximately 50% was demonstrated. Our results suggest that immunization with phylogenetically distant DBL-1α/x variants, can elicit partial cross-protection to challenge with the parasites harbouring a distant variant. These observations have implications for the design of multi-component vaccines against P. falciparum malaria. [Copyright &y& Elsevier]
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Induction of cross-reactive immune responses to NTS-DBL-1α/x of PfEMP1 and in vivo protection on challenge with Plasmodium falciparum
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Autor/in / Beteiligte Person: | Ahuja, Sanjay ; Pettersson, Fredrik ; Moll, Kirsten ; Jonsson, Cathrine ; Wahlgren, Mats ; Chen, Qijun |
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Zeitschrift: | Vaccine, Jg. 24 (2006-08-28), Heft 35/36, S. 6140-6154 |
Veröffentlichung: | 2006 |
Medientyp: | academicJournal |
ISSN: | 0264-410X (print) |
DOI: | 10.1016/j.vaccine.2006.05.030 |
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