Volltext verfügbar nach Anmeldung bzw. im Campus-Netz.
Ischemia-Induced Cleavage of Cadherins in NRK Cells is not Sufficient for β-catenin Transcriptional Activity.
In: Cell Communication & Adhesion, Jg. 14 (2007-10-01), Heft 4, S. 111-123
Online
academicJournal
Zugriff:
Although ischemia is associated with disruption of cadherin-mediated adhesion in renal cell lines, the impact of decreased cadherin function on the transcriptional activity of β-catenin remains poorly defined. In these studies, we used a simulated ischemia model in normal rat kidney (NRK) cells to disrupt cadherin function. Cell viability; cadherin/catenin expression, function, and localization; and β-catenin-mediated transcriptional activity were assessed during ischemia/reperfusion. Following 6 hr of ischemia, a decrease in the expression of E- and N-cadherin was seen that correlated with altered cell morphology indicative of decreased intercellular adhesion. While ischemia was associated with activation of glycogen synthase kinase 3 beta (GSK-3β), this did not correlate with increased phosphorylation of β-catenin as assessed by Western blots using phosphoryl-specific antibodies. β-Catenin was not localized to the nucleus by immunofluorescence in ischemic NRK cells, but rather a strong perinuclear signal was seen in reperfused cells. This was consistent with the finding that neither ischemia nor reperfusion activated the transcriptional activity of β-catenin as assessed by the TCF-optimal promoter (TOPFlash) construct. However, NRK cells possess a competent Wnt pathway, as challenge with lithium chloride elicited a ten-fold increase in luciferase activity. These results suggest that ischemia-induced disruption of cadherin/catenin complexes is not sufficient to stimulate β-catenin transcriptional activity in NRK cells. [ABSTRACT FROM AUTHOR]
Titel: |
Ischemia-Induced Cleavage of Cadherins in NRK Cells is not Sufficient for β-catenin Transcriptional Activity.
|
---|---|
Autor/in / Beteiligte Person: | Chen, Gang ; Akintola, Adebayo D. ; Catania, Jeffrey M. ; Covington, Marisa D. ; Dean, Dana D. ; Trzeciakowski, Jerome P. ; Burghardt, Robert C. ; Parrish, Alan R. |
Link: | |
Zeitschrift: | Cell Communication & Adhesion, Jg. 14 (2007-10-01), Heft 4, S. 111-123 |
Veröffentlichung: | 2007 |
Medientyp: | academicJournal |
ISSN: | 1541-9061 (print) |
DOI: | 10.1080/15419060701556943 |
Schlagwort: |
|
Sonstiges: |
|