Neuroprotective effects of a standardized flavonoid extract from Diospyros kaki leaves
In: Journal of Ethnopharmacology, Jg. 126 (2009-10-29), Heft 1, S. 134-142
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Zugriff:
Abstract: Ethnopharmacological relevance: Flavonoids, extracted from the leaves of Diospyros kaki, are the main therapeutic components of NaoXingQing (NXQ), a potent and patented Chinese herbal remedy widely used in China for the treatment of apoplexy syndrome. Aim of the study: To investigate the neuroprotective effects of FLDK-P70, a standardized flavonoid extract, using in vivo rat models of both focal ischemia/reperfusion (I/R) injury induced by middle cerebral artery occlusion (MCAO) and on transient global brain ischemia induced by four-vessel occlusion (4-VO). We also aim to examine the effects of FLDK-P70 on glutamate-induced cell injury of hippocampal neurons as well as on hypoxia-induced injury of cortical neurons in primary cell culture. Materials and methods and results: Administration of FLDK-P70 for 12 days (40, 80mg/kg body weight, p.o., 5 days before and 7 days after 4-VO) increased the survival of hippocampal CA1 pyramidal neurons after transient global brain ischemia. Similarly, administration of FLDK-P70 for 7 days (40, 80mg/kg body weight, p.o., 3 days before and 4 days after MCAO) significantly reduced the lesion of the insulted brain hemisphere and improved the neurological behavior of rats. In primary rat hippocampal neuronal cultures, pretreatment with FLDK-P70 (5, 10μg/ml) protected neurons from glutamate-induced excitotoxic neuronal death in a dose-dependent manner. In primary rat cerebral cortical neuronal culture, pretreatment with FLDK-P70 (25, 100μg/ml) also reduced hypoxia–reoxygen induced neuronal death and apoptosis in a dose-dependent manner. Conclusions: These in vivo and in vitro results suggest that FLDK-P70 significantly protects rats from MCAO and 4-VO ischemic injury in vivo and protects hippocampal neurons from glutamate-induced excitotoxic injury as well as cortical neurons from hypoxia-induced injury in vitro. The mechanisms of these effects may be due to the antioxidative activity of the flavonoids. These results convincingly demonstrate that FLDK-P70 may be useful for the prevention and treatment of ischemia/reperfusion injury and other related neurodegenerative diseases. [Copyright &y& Elsevier]
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Neuroprotective effects of a standardized flavonoid extract from Diospyros kaki leaves
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Autor/in / Beteiligte Person: | Bei, Weijian ; Zang, Linquan ; Guo, Jiao ; Peng, Wenlie ; Xu, Anlong ; Good, David A. ; Hu, Yinming ; Wu, Wei ; Hu, Dehui ; Zhu, Xinghong ; Wei, Ming ; Li, Chuyuan |
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Zeitschrift: | Journal of Ethnopharmacology, Jg. 126 (2009-10-29), Heft 1, S. 134-142 |
Veröffentlichung: | 2009 |
Medientyp: | academicJournal |
ISSN: | 0378-8741 (print) |
DOI: | 10.1016/j.jep.2009.07.034 |
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