Molecular Cytogenetic Characterization of the Human Cerebral Microvessel Endothelial Cell Line hCMEC/D3.
In: Cytogenetic & Genome Research, Jg. 126 (2010), Heft 4, S. 313-317
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Zugriff:
The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45∼48,XX, –X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13∼14)[2], der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],–22[6][cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies. Copyright © 2009 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
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Molecular Cytogenetic Characterization of the Human Cerebral Microvessel Endothelial Cell Line hCMEC/D3.
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Autor/in / Beteiligte Person: | Mkrtchyan, H. ; Scheler, S. ; Klein, I. ; Fahr, A. ; Couraud, P. O. ; Romero, I. A. ; Weksler, B. ; Liehr, T. |
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Zeitschrift: | Cytogenetic & Genome Research, Jg. 126 (2010), Heft 4, S. 313-317 |
Veröffentlichung: | 2010 |
Medientyp: | academicJournal |
ISSN: | 1424-8581 (print) |
DOI: | 10.1159/000253080 |
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