Changes in the Pattern of Superoxide Dismutase (SOD) and Malate Dehydrogenase (MDH) in Response to Heat Stress Conditions in Wheat.
In: IUP Journal of Genetics & Evolution, Jg. 3 (2010-05-01), Heft 2, S. 23-30
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Zugriff:
High temperature during grain filling stage is the single most important factor that limits the productivity of wheat. Therefore, studies on enzymes that are implicated on thermotolerance during grain development stages are important. Nine homozygous genotypes of wheat (Triticum aestivum L. em. Thell)-PBW 343, WH 283, WH 542, PBW 435, UP 2,565, UP 2,425, EIGN1, EIGN8 and Raj 3,765-and six F1 crosses-PBW 343 × WH 283, PBW 343 × WH 542, PBW 343 × PBW 435, UP 2,565 × UP 2,425, EIGN 1 × Raj 3,765 and EIGN 8 × UP 2,425-were grown in the experimental areas of CCSHAU, Hisar, under normal- and late-sown environments. Changes in protein profiles, based on the banding pattern of isozymes for Superoxide Dismutase (SOD) and Malate Dehydrogenase (MDH), were studied. Based on the banding pattern of SOD isozyme, three isomers of different Rf values, 0.49, 0.75 and 0.85 in normal-sown and 0.84 and 0.87 in late-sown (high temperature condition during grain filling stages), were observed. The band of Rf value 0.75 was found only in the case of EIGN 1 of very dark intensity and light dark intensities in Raj 3,765 and EIGN 8, while in the case of F1s, very dark intensity band in PBW 343 × WH 542 and light dark intensities in PBW 343 × PBW 435 and UP 2,565 × UP 2,425, were observed. Based on the banding pattern of MDH, 4 bands of Rf values, 0.55, 0.67, 0.69 and 0.75 in normal-sown and three bands of Rf values 0.55, 0.75 and 0.82 for late-sown conditions, were observed. The Rf value of 0.82 band was observed only in the case of late-sown among the genotypes PBW 435 (light intensity), UP 2,425, EIGN 1 and EIGN 8 (all three with dark intensity) and F1 crosses PBW 343 × WH 283 (very light intensity, PBW 343 × WH 542 dark intensity), PBW 343 × PBW 435 (dark intensity) and UP 2,565 × UP 2,425 (light intensity). The implications of the results are discussed in the light of the role of enzymes in terminal heat stress in wheat. [ABSTRACT FROM AUTHOR]
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Changes in the Pattern of Superoxide Dismutase (SOD) and Malate Dehydrogenase (MDH) in Response to Heat Stress Conditions in Wheat.
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Autor/in / Beteiligte Person: | Sheikh, Sameena ; Behl, R. K. ; Kumar, Ashwani ; Chabra, A. K. |
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Zeitschrift: | IUP Journal of Genetics & Evolution, Jg. 3 (2010-05-01), Heft 2, S. 23-30 |
Veröffentlichung: | 2010 |
Medientyp: | academicJournal |
ISSN: | 0974-8601 (print) |
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