Surface Plasmon Resonance for Rapid Screening of Uranyl Affine Proteins.
In: Analytical Chemistry, Jg. 82 (2010-12-01), Heft 23, S. 9797-9802
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Zugriff:
A sensitive immunoassay based on SPR analysis was developed to measure uranyl cation (UO22+) affinity for any protein in a free state under physiological conditions. The technique involves immobilization of a specific monoclonal antibody (mAb) raised against UO22+ and 1, 10-phenanthroline-2,9-dicarboxylic acid (DCP) used as a probe of UO22+ captured by the mAb. Calibration curves were established for accurate determination of UO22+ concentrations with a detection limit of 7 nM. The remaining free UO22+ could be accurately quantified from the different protein-metal equilibrium and a dose-response curve established for KD determination. This generic method was applied not only to proteins such as transferrin and albumin but also to small phosphonated ligands. Its robustness allows the fast UO22+ KD determination of any kind of macromolecules and small ligands using very few amount of compounds, thus opening new prospects in the field of uranium toxicity. [ABSTRACT FROM AUTHOR]
Titel: |
Surface Plasmon Resonance for Rapid Screening of Uranyl Affine Proteins.
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Autor/in / Beteiligte Person: | Averseng, Olivier ; Hagege, Agnes ; Taran, Frédéric ; Vidaud, Claude |
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Zeitschrift: | Analytical Chemistry, Jg. 82 (2010-12-01), Heft 23, S. 9797-9802 |
Veröffentlichung: | 2010 |
Medientyp: | academicJournal |
ISSN: | 0003-2700 (print) |
DOI: | 10.1021/ac102578y |
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