A role for L-alpha-lysophosphatidylinositol and GPR55 in the modulation of migration, orientation and polarization of human breast cancer cells.
In: British Journal of Pharmacology, Jg. 160 (2010-06-01), Heft 3, S. 762-771
Online
academicJournal
Zugriff:
Background and Purpose: Increased circulating levels of L-alpha-lysophosphatidylinositol (LPI) are associated with cancer and LPI is a potent, ligand for the G-protein-coupled receptor GPR55. Here we have assessed the modulation of breast cancer cell migration, orientation and polarization by LPI and GPR55. Experimental Approach: Quantitative RT-PCR was used to measure GPR55 expression in breast cancer cell lines. Cell migration and invasion were measured using a Boyden chamber chemotaxis assay and Cultrex invasion assay, respectively. Cell polarization and orientation in response to the microenvironment were measured using slides containing nanometric grooves. Key Results: GPR55 expression was detected in the highly metastatic MDA-MB-231 breast cancer cell line. In these cells, LPI stimulated binding of [(35)S]GTPgammaS to cell membranes (pEC(50) 6.47 +/- 0.45) and significantly enhanced cell chemotaxis towards serum. MCF-7 cells expressed low levels of GPR55 and did not migrate or invade towards serum factors. When GPR55 was over-expressed in MCF-7 cells, serum induced a robust migratory and invasive response, which was further enhanced by LPI and prevented by siRNA to GPR55. The physical microenvironment has been identified as a key factor in determining breast tumour cell metastatic fate. LPI endowed MDA-MB-231 cells with the capacity to detect shallow (40 nm deep) grooved slides and induced marked cancer cell polarization on both flat and grooved surfaces. Conclusions and Implications: LPI and GPR55 play a role in the modulation of migration, orientation and polarization of breast cancer cells in response to the tumour microenvironment. [ABSTRACT FROM AUTHOR]
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A role for L-alpha-lysophosphatidylinositol and GPR55 in the modulation of migration, orientation and polarization of human breast cancer cells.
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Autor/in / Beteiligte Person: | Ford, Lesley A ; Roelofs, Anke J ; Anavi-Goffer, Sharon ; Mowat, Luisa ; Simpson, Daniel G ; Irving, Andrew J ; Rogers, Michael J ; Rajnicek, Ann M ; Ross, Ruth A |
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Zeitschrift: | British Journal of Pharmacology, Jg. 160 (2010-06-01), Heft 3, S. 762-771 |
Veröffentlichung: | 2010 |
Medientyp: | academicJournal |
ISSN: | 0007-1188 (print) |
DOI: | 10.1111/j.1476-5381.2010.00743.x |
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