Flow-Through Microvial Facilitating Interface of Capillary Isoelectric Focusing and Electrospray Ionization Mass Spectrometry.
In: Analytical Chemistry, Jg. 83 (2011-11-15), Heft 22, S. 8748-8755
academicJournal
Zugriff:
A capillary electrophoresis mass spectrometry (CE-MS) interface utilizing a flow-through microvial is used to ensure the electric continuity and supply the catholyte and mobilizer solutions during the capillary isoelectric focusing (cIEF) and mobilization process. The flow-through microvial provides a stable chemical environment and helps to improve the ionization efficiency without significantly diluting the analyte. The CE-MS interface facilitates the transfer of the mobilized cIEF effluent to the site of electrospray ionization, and the gaseous ions can be detected directly by a mass spectrometer. It also allows for complete focusing and mobilization processes to be performed automatically in programmed sequences with commercial CE systems. Two different strategies, using either a part of the capillary or the flow-through microvial of the CE-MS interface as the catholyte reservoir for bare fused silica capillaries or neutral coated capillaries, respectively, were developed for automated cIEF-electrospray ionization (ESI)-MS. Reasonable separation efficiency was achieved using proper concentration of carrier ampholytes and suitable strategies of electroosmotic/electrophoretic mobilization. [ABSTRACT FROM AUTHOR]
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Flow-Through Microvial Facilitating Interface of Capillary Isoelectric Focusing and Electrospray Ionization Mass Spectrometry.
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Autor/in / Beteiligte Person: | Zhong, Xuefei ; Maxwell, E. Jane ; Ratnayake, Chitra ; Mack, Scott ; Chen, David D. Y. |
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Zeitschrift: | Analytical Chemistry, Jg. 83 (2011-11-15), Heft 22, S. 8748-8755 |
Veröffentlichung: | 2011 |
Medientyp: | academicJournal |
ISSN: | 0003-2700 (print) |
DOI: | 10.1021/ac202130f |
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