Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.
In: EMBO Journal, Jg. 31 (2012-05-02), Heft 9, S. 2182-2194
Online
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Zugriff:
The CMG complex composed of Mcm2-7, Cdc45 and GINS is postulated to be the eukaryotic replicative DNA helicase, whose activation requires sequential recruitment of replication proteins onto Mcm2-7. Current models suggest that Mcm10 is involved in assembly of the CMG complex, and in tethering of DNA polymerase ? at replication forks. Here, we report that Mcm10 is required for origin DNA unwinding after association of the CMG components with replication origins in fission yeast. A combination of promoter shut-off and the auxin-inducible protein degradation (off-aid) system efficiently depleted cellular Mcm10 to <0.5% of the wild-type level. Depletion of Mcm10 did not affect origin loading of Mcm2-7, Cdc45 or GINS, but impaired recruitment of RPA and DNA polymerases. Mutations in a conserved zinc finger of Mcm10 abolished RPA loading after recruitment of Mcm10. These results show that Mcm10, together with the CMG components, plays a novel essential role in origin DNA unwinding through its zinc-finger function. [ABSTRACT FROM AUTHOR]
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Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.
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Autor/in / Beteiligte Person: | Kanke, Mai ; Kodama, Yukako ; Takahashi, Tatsuro S ; Nakagawa, Takuro ; Masukata, Hisao |
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Zeitschrift: | EMBO Journal, Jg. 31 (2012-05-02), Heft 9, S. 2182-2194 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 0261-4189 (print) |
DOI: | 10.1038/emboj.2012.68 |
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