Voltammetric studies of the interaction of transition-metal complexes with DNA
In: Journal of Biochemical & Biophysical Methods, Jg. 52 (2002-08-01), Heft 3, S. 189-200
academicJournal
Zugriff:
The interaction of the two new synthesized transition-metal complexes, ML2 (M=Co, Cu, L=1,8-dihydroxyethyl-1, 3,8,10,13-hexa-azacyclotetradecane) with calf thymus DNA was probed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Adding deoxyribonucleic acid (DNA) into [CoL]2+ and [CuL]2+ solution, the ip value of all the peaks of [CoL]2+ and [CuL]2+ significantly decreased in proportion to concentration of DNA. Glassy carbon electrodes (GCEs) were modified with DNA by adsorption, and it was electrochemically characterized with transition-metal complexes, [ML]2+. The DNA modification layer on the GCE is unstable to alkali and to heat, but stable to acid solutions and very stable in long stock in a dry state. It could be seen that peak potential shifted positively and the peak current increased significantly. The electrochemical parameters, binding constant (kn+) and binding sites(s) were calculated by a nonlinear regression method. [Copyright &y& Elsevier]
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Voltammetric studies of the interaction of transition-metal complexes with DNA
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Autor/in / Beteiligte Person: | Lu, Xiaoquan ; Zhu, Kaimei ; Zhang, Min ; Liu, Hongde ; Kang, Jingwan |
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Zeitschrift: | Journal of Biochemical & Biophysical Methods, Jg. 52 (2002-08-01), Heft 3, S. 189-200 |
Veröffentlichung: | 2002 |
Medientyp: | academicJournal |
ISSN: | 0165-022X (print) |
DOI: | 10.1016/S0165-022X(02)00074-X |
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