Urotensin II promotes the production of LTC in rat aortic adventitial fibroblasts through NF-κB-5-LO pathway by p38 MAPK and ERK activations.
In: Heart & Vessels, Jg. 28 (2013-07-01), Heft 4, S. 514-523
Online
academicJournal
Zugriff:
Adventitia is the outer part of the arterial wall where the inflammatory response often occurs. Urotensin II (UII) is a potent vasoconstrictive peptide that also promotes the inflammatory process in patients with cardiovascular disease. Leukotriene C (LTC), a lipid mediator, was recently found to play a role in the inflammatory process in the artery. We hypothesized that the adventitia is one of the resources of LTC and that UII may promote LTC production through the 5-LO (5-lipoxygenase) pathway in adventitial fibroblasts. Rat adventitial fibroblasts were isolated and incubated in serum-free medium with either UII alone or in combination with inhibitors of p38 MAPK, ERK, and UII receptors. The expression of 5-LO was detected using real-time polymerase chain reaction and Western blot. The translocation and binding activity of nuclear factor (NF)-κB were measured using immunofluorescence and electrophoretic mobility shift assay, respectively. The production of LTC was measured by enzyme-linked immunosorbent assay. The results indicated that: (1) adventitial fibroblasts were a source of LTC production; (2) UII increased the expression of the 5-LO mRNA and the protein by NF-κB activation through p38 MAPK and ERK pathways; and (3) UII promoted the LTC release in fibroblasts through the 5-LO pathway by p38 MAPK and ERK activations. The 5-LO pathway mediates LTC production, which may be a new mechanism in the pathogenesis of the vascular adventitial inflammation caused by UII. [ABSTRACT FROM AUTHOR]
Titel: |
Urotensin II promotes the production of LTC in rat aortic adventitial fibroblasts through NF-κB-5-LO pathway by p38 MAPK and ERK activations.
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Autor/in / Beteiligte Person: | Dong, Xiao ; Ye, Xiaojin ; Song, Nana ; Zhao, Jing ; Di, Beibing ; Peng, Fen ; Tang, Chaoshu ; Ding, Wenhui |
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Zeitschrift: | Heart & Vessels, Jg. 28 (2013-07-01), Heft 4, S. 514-523 |
Veröffentlichung: | 2013 |
Medientyp: | academicJournal |
ISSN: | 0910-8327 (print) |
DOI: | 10.1007/s00380-012-0291-0 |
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