High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture.
In: Biomaterials, Jg. 34 (2013-11-10), Heft 34, S. 8555-8563
academicJournal
Zugriff:
Abstract: Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method based on a magnetic pin-array system to concentrate magnetic nanoparticle-incorporated cells in a focal direction. This system was comprised only of external magnets and magnetically induced iron pins to generate a concentrated magnetic field for attracting cells in a focused direction. 3-D spheroid generation was achieved simply by adding magnetic nanoparticle-incorporated cells into a well and covering the plate with a magnetic lid. Cell clustering occurred rapidly within 5 min and created more compact cells with time through the focused magnetic force. This system ensured not only reproducible and size-controlled generation of spheroids but also versatile types of spheroids such as random mixed, core–shell, and fused spheroids, providing a very useful tool for various biological applications. [Copyright &y& Elsevier]
Titel: |
High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture.
|
---|---|
Autor/in / Beteiligte Person: | Kim, Jeong Ah ; Choi, Jong-Ho ; Kim, Minsoo ; Rhee, Won Jong ; Son, Boram ; Jung, Hyun-Kyo ; Park, Tai Hyun |
Link: | |
Zeitschrift: | Biomaterials, Jg. 34 (2013-11-10), Heft 34, S. 8555-8563 |
Veröffentlichung: | 2013 |
Medientyp: | academicJournal |
ISSN: | 0142-9612 (print) |
DOI: | 10.1016/j.biomaterials.2013.07.056 |
Schlagwort: |
|
Sonstiges: |
|