cIEF for rapid pKa determination of small molecules: A proof of concept.
In: European Journal of Pharmaceutical Sciences, Jg. 63 (2014-10-15), S. 14-21
academicJournal
Zugriff:
A capillary isoelectric focusing (cIEF) method was developed for the determination of the ionization constants (p K a ) of small molecules. Two approaches used to decrease the electroosmotic flow (EOF) were compared: (i) a hydroxypropylcellulose (HPC) coated capillary in aqueous medium and (ii) the addition of glycerol to act as a viscosifying agent. The cIEF method with the glycerol medium was selected, and the ionization constants of 22 basic and 21 acidic compounds, including 15 pharmaceutical drugs, were determined, resulting in p K a values from 3.5 to 7.4 and 6.4 to 9.3, respectively. cIEF offers an interesting alternative to other techniques for p K a determination with low sample consumption, high throughput and low cost. [ABSTRACT FROM AUTHOR]
Titel: |
cIEF for rapid pKa determination of small molecules: A proof of concept.
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Autor/in / Beteiligte Person: | Romand, Stéphanie ; Schappler, Julie ; Veuthey, Jean-Luc ; Carrupt, Pierre-Alain ; Martel, Sophie |
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Zeitschrift: | European Journal of Pharmaceutical Sciences, Jg. 63 (2014-10-15), S. 14-21 |
Veröffentlichung: | 2014 |
Medientyp: | academicJournal |
ISSN: | 0928-0987 (print) |
DOI: | 10.1016/j.ejps.2014.06.016 |
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