Bordetella bronchiseptica-mediated cytotoxicity to macrophages is dependent on bvg-regulated factors, including pertactin.
In: Infection and immunity, Jg. 67 (1999-11-01), Heft 11, S. 5972-8
Online
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Zugriff:
The effect of Bordetella bronchiseptica infection on the viability of murine macrophage-like cells and on primary porcine alveolar macrophages was investigated. The bacterium was shown to be cytotoxic for both cell types, particularly where tight cell-to-cell contacts were established. In addition, bvg mutants were poorly cytotoxic for the eukaryotic cells, while a prn mutant was significantly less toxic than wild-type bacteria. B. bronchiseptica-mediated cytotoxicity was inhibited in the presence of cytochalasin D or cycloheximide, an inhibitor of microfilament-dependent phagocytosis or de novo eukaryotic protein synthesis, respectively. The mechanism of eukaryotic cell death was examined, and cell death was found to occur primarily through a necrotic pathway, although a small proportion of the population underwent apoptosis.
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Bordetella bronchiseptica-mediated cytotoxicity to macrophages is dependent on bvg-regulated factors, including pertactin.
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Autor/in / Beteiligte Person: | Forde, CB ; Shi, X ; Li, J ; Roberts, M |
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Zeitschrift: | Infection and immunity, Jg. 67 (1999-11-01), Heft 11, S. 5972-8 |
Veröffentlichung: | Washington, DC : American Society For Microbiology ; <i>Original Publication</i>: [Bethesda, Md.] American Society for Microbiology., 1999 |
Medientyp: | academicJournal |
ISSN: | 0019-9567 (print) |
DOI: | 10.1128/IAI.67.11.5972-5978.1999 |
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