[Expression and reversion of drug resistance- and apoptosis-related genes of a DDP-resistant lung adenocarcinoma cell line A549DDP].

Wang, J ; Liu, X ; et al.

In: Zhonghua zhong liu za zhi [Chinese journal of oncology], Jg. 21 (1999-11-01), Heft 6, S. 422-6

academicJournal

Objective: To study the expression of drug resistance- and apoptosis-related genes of A549DDP cells as compared to the parental cell line A549, and its reversion by antisense s-oligodeoxynucleotide (S-ODN) of the differentially expressed genes.

Methods: Sense and antisense S-ODN were transferred into A549DDP cells by lipofectin. Expression of genes related to drug resistance and apoptosis was examined by RT-PCR, immunocytochemistry and flow cytometry. Apoptosis was identified by DNA electrophoresis and TUNEL, and cell growth by MTT uptake.

Results: The expression of bcl-2 was positive and that of MRP at mRNA and protein levels was increased in A549DDP cells compared to A549 cells. MDR1, c-myc and TOPO II were similarly expressed in the two cell lines. Both cell lines were negative for c-erbB-2 expression. In A549DDP cells, the expression of bcl-2 and MRP was significantly inhibited by respective antisense S-ODN. Antisense S-ODN could also significantly inhibit proliferation of A549DDP cells, and promote cell apoptosis by reducing its resistance to cisplatin.

Conclusion: Bcl-2 and MRP genes are responsible for the induced resistance of A549DDP cells to cisplatin.

Titel:	[Expression and reversion of drug resistance- and apoptosis-related genes of a DDP-resistant lung adenocarcinoma cell line A549DDP].
Autor/in / Beteiligte Person:	Wang, J ; Liu, X ; Wu, M
Zeitschrift:	Zhonghua zhong liu za zhi [Chinese journal of oncology], Jg. 21 (1999-11-01), Heft 6, S. 422-6
Veröffentlichung:	Peking : Chinese Medical Association ; <i>Original Publication</i>: Beijing, Zhonghua yi xue hui., 1999
Medientyp:	academicJournal
ISSN:	0253-3766 (print)
Schlagwort:	Adenocarcinoma genetics Adenocarcinoma pathology Genes, bcl-2 Genes, myc Humans Lung Neoplasms genetics Lung Neoplasms pathology Multidrug Resistance-Associated Proteins genetics RNA, Messenger analysis Tumor Cells, Cultured Adenocarcinoma drug therapy Apoptosis genetics Drug Resistance, Neoplasm genetics Lung Neoplasms drug therapy
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: Chinese Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't Language: Chinese [Zhonghua Zhong Liu Za Zhi] 1999 Nov; Vol. 21 (6), pp. 422-6. MeSH Terms: Adenocarcinoma / drug therapy ; Apoptosis / genetics ; Drug Resistance, Neoplasm / genetics ; Lung Neoplasms / drug therapy ; Adenocarcinoma / genetics ; Adenocarcinoma / pathology ; Genes, bcl-2 ; Genes, myc ; Humans ; Lung Neoplasms / genetics ; Lung Neoplasms / pathology ; Multidrug Resistance-Associated Proteins / genetics ; RNA, Messenger / analysis ; Tumor Cells, Cultured Substance Nomenclature: 0 (Multidrug Resistance-Associated Proteins) ; 0 (RNA, Messenger) Entry Date(s): Date Created: 20020105 Date Completed: 20020207 Latest Revision: 20061115 Update Code: 20240513

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